Low-Toxicity Solvents for the Extraction of Valuable Lipid Compounds from Octopus (Octopus vulgaris) Waste

This study focused on the recovery of valuable lipid compounds from octopus (Octopus vulgaris) by-products. Extraction conditions of total lipids (TLs), phospholipids (PLs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) were optimized by employing a Simplex-Lattice design; for it, different relative concentrations of three low-toxicity solvents (ethanol, acetone, and ethyl acetate) were considered. The optimization process was also addressed in reference to fatty acid (FA) ratios (total polyunsaturated FAs/total saturated FAs and total omega 3 FAs/total omega 6 FAs). The variance analysis of multiple regression data demonstrated that the quadratic model was significant (p < 0.05) for TL, PL, and DHA values and the omega 3/omega 6 ratio. As a result, the following optimized values were obtained: 113.8 g.kg(-1) dry by-products (TLs), 217.3 g.kg(-1) lipids (PLs), 22.55 g.100 g(-1) total FAs (DHA), and 3.70 (omega 3/omega 6 ratio). According to the model developed, optimized values were shown to correspond to the following relative solvent concentrations (ethanol/acetone/ethyl acetate): 0.46/0.00/0.54, 0.93/0.07/0.00, 0.83/0.17/0.00, and 0.64/0.00/0.36, respectively. Comparison to yields obtained by the conventional chloroform/methanol method was carried out. A novel strategy based on the employment of low-toxicity solvents is proposed for the extraction of valuable lipid constituents from octopus waste. A different solvent ratio would be necessary according to the lipid compound concerned.

Automated summary

This study focused on optimizing the extraction conditions of valuable lipid compounds from octopus by-products using a Simplex-Lattice design and low-toxicity solvents. The results showed that the quadratic model was significant for the extraction of total lipids, phospholipids, and docosahexaenoic acid (DHA), as well as the omega 3/omega 6 ratio. The optimized values and solvent concentrations were determined, and a comparison to conventional methods was made. The study proposed a novel strategy for the extraction of valuable lipid constituents from octopus waste using low-toxicity solvents.