期刊
NATURE MATERIALS
卷 15, 期 12, 页码 1287-1296出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMAT4729
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资金
- NIH [R01 GM072744, R01 GM58460]
- Huazhong University of Science and Technology
- Ministry of Science and Technology of China [2016YFA0101100]
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- NSF IGERT
- Hoeft Professorship at University of Illinois at Urbana-Champaign
Mechanical forces play critical roles in the function of living cells. However, the underlying mechanisms of how forces influence nuclear events remain elusive. Here, we show that chromatin deformation as well as force-induced transcription of a green fluorescent protein (GFP)-tagged bacterial-chromosome dihydrofolate reductase (DHFR) transgene can be visualized in a living cell by using three-dimensional magnetic twisting cytometry to apply local stresses on the cell surface via an Arg-Gly-Asp-coated magnetic bead. Chromatin stretching depended on loading direction. DHFR transcription upregulation was sensitive to load direction and proportional to the magnitude of chromatin stretching. Disrupting filamentous actin or inhibiting actomyosin contraction abrogated or attenuated force-induced DHFR transcription, whereas activating endogenous contraction upregulated force-induced DHFR transcription. Our findings suggest that local stresses applied to integrins propagate from the tensed actin cytoskeleton to the LINC complex and then through lamina-chromatin interactions to directly stretch chromatin and upregulate transcription.
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