期刊
NATURE GENETICS
卷 48, 期 6, 页码 687-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ng.3555
关键词
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资金
- Agriculture and Agri-Food Canada A-base
- National Science and Engineering Research Council of Canada [R4019A01]
- Natural Science Foundation of China [31128001, 31210103901]
- State Key Laboratory of Plant Genomics [2015B0129-01]
- US National Institutes of Health [GM061146, R01GM066258]
- Chinese Scholarship Council
SWI/SNF-type chromatin remodelers, such as BRAHMA (BRM), and H3K27 demethylases both have active roles in regulating gene expression at the chromatin level(1-5), but how they are recruited to specific genomic sites remains largely unknown. Here we show that RELATIVE OF EARLY FLOWERING 6 (REF6), a plant-unique H3K27 demethylase(6), targets genomic loci containing a CTCTGYTY motif via its zinc-finger (ZnF) domains and facilitates the recruitment of BRM. Genome-wide analyses showed that REF6 colocalizes with BRM at many genomic sites with the CTCTGYTY motif. Loss of REF6 results in decreased BRM occupancy at BRM-REF6 co-targets. Furthermore, REF6 directly binds to the CTCTGYTY motif in vitro, and deletion of the motif from a target gene renders it inaccessible to REF6 in vivo. Finally, we show that, when its ZnF domains are deleted, REF6 loses its genomic targeting ability. Thus, our work identifies a new genomic targeting mechanism for an H3K27 demethylase and demonstrates its key role in recruiting the BRM chromatin remodeler.
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