Biological Activity and Metabolomics of Griffonia simplicifolia Seeds Extracted with Different Methodologies

Griffonia simplicifolia, a tropical plant endemic to West Africa, is highly regarded for its significant pharmacological potential. The objective of this study was to evaluate the metabolomic profile and to explore the antioxidant properties, antiproliferative activity, and antimicrobial potential of G. simplicifolia seed extracts obtained through either maceration, microwave-assisted extraction (MAE), or Soxhlet extraction using water, acetone, methanol and ethanol as solvents. Overall, methanol possessed superior total extraction efficiency. HPLC analyses confirmed the efficacy of acetone and ethanol as optimal solvents for the extraction of flavonoids and flavan-3-ols, whereas MAE exhibited enhanced effectiveness in extracting N-containing compounds, including 5-hydroxytryptophan (5-HTP). HPLC-MS analyses identified forty-three compounds, including thirty-four phenolic compounds and nine N-containing molecules. Isomyricitrin, taxifolin and a flavonol glucuronide were the main polyphenols, whereas 5-HTP was the main N-containing compound. Hydroalcoholic G. simplicifolia extracts showed the highest radical scavenging and metal-reducing antioxidant power, suggesting that most of the contribution to antioxidant activity depends on the more polar bioactive compounds. G. simplicifolia extracts showed dose-dependent antiproliferative activity against three distinct cancer cell lines (HeLa, HepG2, and MCF-7), with notable variations observed among both the different extracts and cell lines and divergent GI(50) values, emphasizing substantial discrepancies in cell sensitivity to the various extracts. Furthermore, G. simplicifolia extracts revealed antibiotic activity against Staphylococcus aureus. Our results highlight the potential of G. simplicifolia phytochemicals in the development of functional foods, nutraceuticals, and dietary supplements.

Automated summary

This study evaluated the metabolomic profile and explored the antioxidant properties, antiproliferative activity, and antimicrobial potential of Griffonia simplicifolia seed extracts. The results showed that methanol had superior extraction efficiency, while acetone and ethanol were effective solvents for flavonoid extraction. Microwave-assisted extraction (MAE) was found to be effective in extracting N-containing compounds. The extracts exhibited significant antioxidant and antiproliferative activity, with variations observed among different extracts and cell lines. Griffonia simplicifolia extracts also showed antibiotic activity against Staphylococcus aureus. The findings highlight the potential of Griffonia simplicifolia phytochemicals in the development of functional foods, nutraceuticals, and dietary supplements.