Hemocompatibility of beta-Cyclodextrin-Modified (Methacryloyloxy)ethyl Phosphorylcholine Coated Magnetic Nanoparticles

Adsorbing toxins from the blood to augment membrane-based hemodialysis is an active area of research. Films composed of beta-cyclodextrin-co-(methacryloyloxy)ethyl phosphorylcholine (p(PM fiCD-co-MPC)) with various monomer ratios were formed on magnetic nanoparticles and characterized. Surface chemistry effects on protein denaturation were evaluated and indicated that unmodified magnetic nanoparticles greatly perturbed the structure of proteins compared to coated particles. Plasma clotting assays were conducted to investigate the stability of plasma in the presence of particles, where a 2:2 monomer ratio yielded the best results for a given total surface area of particles. Total protein adsorption results revealed that modified surfaces exhibited reduced protein adsorption compared to bare particles, and pure MPC showed the lowest adsorption. Immunoblot results showed that fibrinogen, ff1-antitrypsin, vitronectin, prekallikrein, antithrombin, albumin, and C3 correlated with film composition. Hemocompatibility testing with whole blood illustrated that the 1:3 ratio of CD to MPC had a negative impact on platelets, as evidenced by the increased activation, reduced response to an agonist, and reduced platelet count. Other formulations had statistically significant effects on platelet activation, but no formulation yielded apparent adverse effects on hemostasis. For the first time, p(PM fiCD-co-MPC)-coated MNP were synthesized and their general hemocompatibility assessed.

Automated summary

Adsorbing toxins from blood to enhance hemodialysis is a trending research area. Films composed of p(PM fiCD-co-MPC)-coated magnetic nanoparticles were investigated for their effects on protein denaturation and plasma stability. The modified surfaces exhibited reduced protein adsorption and the ratio of CD to MPC had a negative impact on platelets.