期刊
NATURE CHEMISTRY
卷 8, 期 7, 页码 698-704出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.2517
关键词
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资金
- National Natural Science Foundation of China [31470532, 91543102, 21532004]
- Ministry of Science and Technology of China [2015CB553402, 2013CB932800]
- Tsinghua University Initiative Scientific Research Program
- Tsinghua University-Peking University Center for Life Sciences
- Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases
The overwhelmingly homochiral nature of life has left a puzzle as to whether mirror-image biological systems based on a chirally inverted version of molecular machinery could also have existed. Here we report that two key steps in the central dogma of molecular biology, the template-directed polymerization of DNA and transcription into RNA, can be catalysed by a chemically synthesized D-amino acid polymerase on an L-DNA template. We also show that two chirally mirrored versions of the 174-residue African swine fever virus polymerase X could operate in a racemic mixture without significant enantiomeric cross-inhibition to the activity of each other. Furthermore, we demonstrate that a functionally active L-DNAzyme could be enzymatically produced using the D-amino acid polymerase. The establishment of such molecular systems with an opposite handedness highlights the potential to exploit enzymatically produced mirror-image biomolecules as research and therapeutic tools.
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