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Review
Multidisciplinary Sciences
Joy Y. Wang et al.
Summary: The combination of CRISPR genome editing technology and advances in computing and imaging capabilities has brought about a new era in which genetic diseases and individual disease susceptibilities can be predicted and acted upon. This technology has also revolutionized agricultural research and plant breeding by enabling quick identification and alteration of genes responsible for plant traits. In this Review, we discuss the current state of CRISPR-mediated genetic manipulation in human cells, animals, and plants, highlighting successes, challenges, and future prospects.
Editorial Material
Biotechnology & Applied Microbiology
Yaru Li et al.
Summary: This study aims to compare the similarities, differences, and complementarities between the CRISPR/Cas system and Argonaute (Ago) as nucleases, in order to guide the design of novel nucleic acid tests.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Yaru Li et al.
Summary: Foodborne pathogenic bacteria are major causes of microbial contamination in food safety, and early screening and ultrasensitive detection are crucial for guaranteeing food safety. In this study, a Novel and One-step cleavage method based on Argonaute (NOTE-Ago) was developed, which allowed target nucleic acid to be cleverly converted into fluorescent signals. The NOTE-Ago assay exhibited a detection limit of 1 CFU/mL and a dynamic range from 1 to 108 CFU/mL, with satisfactory selectivity for detecting S. typhi and S. aureus contaminated food samples. This work enriches the toolbox of Argonaute-based detection and provides a one-step cleavage and rebuilding-free method for ultrasensitive bacteria detection.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Analytical
Qiuyuan Lin et al.
Summary: The study presents a general and programmable diagnostic strategy for miRNA analysis. By combining exponential isothermal amplification and programmable TtAgo cleavage, this method enables rapid and sensitive multiplex detection of miRNAs.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Shiying Zhou et al.
Summary: The proposed method combines endonucleases, CRISPR/Cas, and recombinases to detect DNA methylation with high sensitivity. It shows great potential for the analysis of tumor-specific genes.
Article
Biophysics
Fei Wang et al.
Summary: In this study, a highly sensitive and accurate molecular diagnosis platform named SARS-CoV-2 PAND was developed for large-scale screening of COVID-19 infection. The use of Pyrococcus furiosus Argonaute technology allowed for rapid detection within an hour, significantly reducing the time needed for Real-time fluorescence quantitative PCR instrument usage. This platform showed 100% consistency with RT-qPCR in diagnosing clinic samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Eric A. Hunt et al.
Summary: Research on the Argonaute protein from Thermus thermophilus has revealed specific preferences for guide sequences, which can be used to enhance guide design for molecular applications. Notable preferences include a strong cleavage enhancement from a first position dT independent of target sequence, a significant decrease in activity with dA at position 12, and an impact of GC dinucleotides at positions 10 and 11. This approach could potentially be extended to characterize other variants and improve their utility in biology and genome editing applications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Senyao Wang et al.
Summary: A hierarchically porous Zr-MOE-labeled electrochemical aptasensor utilizing PtPd@Ni-Co hollow nanoboxes and poly (diallyldimethylammonium chloride)-functionalized graphene was developed for ultrasensitive chloramphenicol detection. By incorporating hierarchically porous Zr-MOFs as signal probes and employing Exo III-assisted cyclic amplification strategies, the aptasensor demonstrated satisfactory sensitivity in a wide linear range and a low detection limit. The signal amplification strategies employed in this study effectively improved sensor performance, offering a new avenue for the development of ultrasensitive sensors in food analysis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Engineering, Biomedical
Michael M. Kaminski et al.
Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Jinzhao Song et al.
NUCLEIC ACIDS RESEARCH
(2020)
Article
Chemistry, Analytical
Shuang Liu et al.
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Soochul Shin et al.
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Lei Chang et al.
NANOSCALE HORIZONS
(2019)
Review
Biochemistry & Molecular Biology
Frank Hille et al.
Article
Multidisciplinary Sciences
Audrone Lapinaite et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Review
Biochemistry & Molecular Biology
Zhenqiang Sun et al.
Article
Biochemistry & Molecular Biology
Gang Sheng et al.
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemical Research Methods
Behnam Enghiad et al.
ACS SYNTHETIC BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Daan C. Swarts et al.
