Longitudinal anellome dynamics in the upper respiratory tract of children with acute respiratory tract infections

Anelloviruses (AVs) are ubiquitous in humans and are the most abundant components of the commensal virome. Previous studies on the diversity, transmission, and persistence of AVs mainly focused on the blood or transplanted tissues from adults; however, the profile of the anellome in the respiratory tract in children are barely known. We investigated the anellome profile and their dynamics in the upper respiratory tract from a cohort of children with acute respiratory tract infections (ARTIs). Different to that in adult, betatorquevirus is the most abundant genus, followed by alphatorquevirus. We found that the relative abundance of betatorquevirus was higher in earlier time points, and in contrast, the abundance of alphatorquevirus was higher in later time points; these results might suggest that betatorquevirus decreased with age and alphatorquevirus increased with age in childhood. No difference regarding the diversity and abundance of anellome was found between single and multiple ARTIs, consistent with the idea that AV is not associated with certain disease. Most AVs are transient, and a small proportion (8 per cent) of them were found to be possibly persistent, with persistence time ranging from 1 month to as long as 56 months. Furthermore, the individual respiratory anellome appeared to be unique and dynamic, and the replacement of existing AVs with new ones are common over different time points. These findings demonstrate that betatorquevirus may be the early colonizer in children, and the individual respiratory anellome is unique, which are featured by both chronic infections and AV community replacement.

Automated summary

Anelloviruses (AVs) are abundant in humans and are the most prevalent components of the commensal virome. Research on AV diversity, transmission, and persistence has mainly focused on adults, with limited understanding of the anellome in the respiratory tract of children. This study investigated the anellome profile and dynamics in the upper respiratory tract of children with acute respiratory tract infections (ARTIs). The findings suggest that betatorquevirus may decrease with age, while alphatorquevirus increases with age in childhood, and the individual respiratory anellome is unique and undergoes common replacement over time.