期刊
GUT MICROBES
卷 15, 期 2, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19490976.2023.2252447
关键词
Lactobacillus johnsonii N6.2; probiotic; microbial-derived lipids; phospholipid; innate immunity; dendritic cell; cellular immune response; immunomodulation
Shifts in the gut microbiota composition, called dysbiosis, have been associated with diseases and their underlying biological systems are not well understood. Phospholipids act as precursors to inflammatory and resolving mediators and their dysregulation is linked to chronic diseases. Gut microbial-derived lipids have unique structures and can modulate host immunity. Lactobacillus johnsonii N6.2 is a probiotic symbiont that reduces autoimmunity and promotes anti-inflammatory responses.
Shifts in the gut microbiota composition, called dysbiosis, have been directly associated with acute and chronic diseases. However, the underlying biological systems connecting gut dysbiosis to systemic inflammatory pathologies are not well understood. Phospholipids (PLs) act as precursors of both, bioactive inflammatory and resolving mediators. Their dysregulation is associated with chronic diseases including cancer. Gut microbial-derived lipids are structurally unique and capable of modulating host's immunity. Lactobacillus johnsonii N6.2 is a Gram-positive gut symbiont with probiotic characteristics. L. johnsonii N6.2 reduces the incidence of autoimmunity in animal models of Type 1 Diabetes and improves general wellness in healthy volunteers by promoting, in part, local and systemic anti-inflammatory responses. By utilizing bioassay-guided fractionation methods with bone marrow-derived dendritic cells (BMDCs), we report here that L. johnsonii N6.2 purified lipids induce a transcriptional signature that resembles that of migratory (mig) DCs. RNAseq-based analysis showed that BMDCs stimulated with L. johnsonii N6.2 total lipids upregulate maturation-mig related genes Cd86, Cd40, Ccr7, Icam1 along with immunoregulatory genes including Itgb8, Nfkbiz, Jag1, Adora2a, IL2ra, Arg1, and Cd274. Quantitative reverse transcription (qRT)-PCR analysis indicated that PLs are the bioactive lipids triggering the BMDCs response. Antibody-blocking of surface Toll-like receptor (TLR)2 resulted in boosted PL-mediated upregulation of pro-inflammatory Il6. Chemical inhibition of the IKKa kinase from the non-canonical NF-?B pathway specifically restricted upregulation of Il6 and Tnf. Phenotypically, PL-stimulated BMDCs displayed an immature like-phenotype with significantly increased surface ICAM-1. This study provides insight into the immunoregulatory capacity of Gram-positive, gut microbial-derived phospholipids on innate immune responses.
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