期刊
NATURE CHEMICAL BIOLOGY
卷 12, 期 6, 页码 393-U29出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.2062
关键词
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资金
- US DOE grant [DE-FC02-02ER63421]
- ARPA-E grant [DE-AR0000556]
- U.S. Department of Energy (DOE) [DE-FC02-02ER63421] Funding Source: U.S. Department of Energy (DOE)
Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.
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