期刊
NATURE CHEMICAL BIOLOGY
卷 13, 期 1, 页码 12-14出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.2226
关键词
-
资金
- Cancer Research UK (CRUK) [C6/A11224]
- European Community Seventh Framework Programme [HEALTH -F2-2010-259893]
- Cancer Research UK [C6946/A14492]
- Wellcome Trust [WT092096]
- Cancer Research UK programme [C6/A11224]
- Ataxia Telangiectasia Society
- CRUK
- European Research Council under the European Union's Seventh Framework Programme
- Boehringer Ingelheim Fonds PhD fellowship
- European Research Council
- University of Cambridge
- ERC [311166]
- Cancer Research UK [18796, 21717, 13031] Funding Source: researchfish
- European Research Council (ERC) [311166] Funding Source: European Research Council (ERC)
In model organisms, classical genetic screening via random mutagenesis provides key insights into the molecular bases of genetic interactions, helping to define synthetic lethality, synthetic viability and drug-resistance mechanisms. The limited genetic tractability of diploid mammalian cells, however, precludes this approach. Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据