期刊
NATURE BIOTECHNOLOGY
卷 35, 期 1, 页码 31-34出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3737
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资金
- Human Frontiers Scientific Program
- Paul and Daisy Soros Fellowship
- Friends of the McGovern Institute Fellowship
- D.O.E. Computational Science Graduate Fellowship
- National Institute of Biomedical Imaging and Bioengineering (NIBIB), of the National Institutes of Health [5T32EB1680]
- NIH [GM10407]
- Russian Science Foundation [14-14-00988]
- Skoltech
- Netherlands Organization for Scientific Research (NWO) through a TOP grant [714.015.001]
- NIH through NIMH [5DP1-MH100706, 1R01-MH110049]
- New York Stem Cell Foundation
- Poitras Foundation
- Simons Foundation
- Paul G. Allen Family Foundation
- Vallee Foundation
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [T32EB001680] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM104071] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [DP1MH100706, R01MH110049] Funding Source: NIH RePORTER
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpfl to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
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