期刊
NATURE BIOTECHNOLOGY
卷 34, 期 6, 页码 652-655出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3543
关键词
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资金
- Gatsby Charitable Foundation, UK
- Two Blades Foundation, USA
- Biotechnology and Biological Sciences Research Council, UK
- Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project
- Bill & Melinda Gates Foundation
- UK Department for International Development
- USDA-ARS National Plant Disease Recovery System
- Grains Research and Development Corporation, Australia
- Universiti Putra Malaysia (UPM), Malaysia
- NBI Computing infrastructure for Science (CiS) group
- ARS [ARS-0423040, 813313] Funding Source: Federal RePORTER
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0965429] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/M003809/1, BB/L011794/1, BB/L009293/1, BB/H019820/1, BB/J003166/1, BBS/E/J/000C0673, BBS/E/J/000CA562] Funding Source: researchfish
- BBSRC [BB/H019820/1, BBS/E/J/000C0673, BB/M003809/1, BB/J003166/1, BBS/E/J/000CA562, BB/L009293/1, BB/L011794/1] Funding Source: UKRI
Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution(1). If several cloned R genes were available, it would be possible to pyramid R genes(2) in a crop, which might provide more durable resistance(1). We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
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