期刊
NATURE BIOTECHNOLOGY
卷 34, 期 9, 页码 987-+出版社
NATURE RESEARCH
DOI: 10.1038/nbt.3625
关键词
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资金
- MIT Media Lab
- MIT Brain and Cognitive Sciences Department
- New York Stem Cell Foundation-Robertson Investigator Award
- National Institutes of Health (NIH) Transformative Award [1R01GM104948]
- NIH Director's Pioneer Award [1DP1NS087724, NIH 1R01EY023173, NIH 1U01MH106011]
- MIT McGovern Institute
- Open Philanthropy Project
- National Science Foundation fellowship
- Poitras Fellowship
- Integrative Neuronal Systems [T32 GM007484]
- Hertz Fellowship
- Howard Hughes Medical Institute
- MIT McGovern Institute MINT program
- Grants-in-Aid for Scientific Research [16K10787] Funding Source: KAKEN
Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (similar to 70 nm) imaging of cells and mammalian tissues on conventional microscopes.
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