Scoparia dulcis L. Extract Relieved High Stocking Density-Induced Stress in Crucian Carp (Carassius auratus)

Simple Summary High stocking density and Cu and trichlorfon exposure are typical inducers of physiological stress in fish. It is well known that the stress response may be harmful to aquatic animals, inhibiting growth, causing reproductive failure, and reducing resistance to pathogens. Hence, it is important to attenuate the detrimental effects induced by these stressors in modern farming systems. Although Scoparia dulcis relieved cadmium-induced oxidative stress in rats, little information is available on the effects of high stocking density and CuSO4 and trichlorfon exposure in fish. Therefore, this study investigates the protective effects of dietary supplementation with Scoparia dulcis extract on fish maintained at a high stocking density and exposed to CuSO4 and trichlorfon. The present study may offer a feasible way of relieving the effects induced by these stressors. The objective of this study was to investigate the effects of Scoparia dulcis extract (SDE) on stress induced by high stocking density and Cu and trichlorfon exposure in crucian carp (Carassius auratus). The results showed that these stressors exerted detrimental effects in fish, such as inhibition of growth performance, reduced feed intake, and interruption of fish locomotion. Under high stocking density, dietary SDE supplementation increased the content of reduced glutathione (GSH) and the activities of amylase, catalase (CAT), and glutathione reductase (GR) and decreased the content of malonaldehyde (MDA) in the intestine of crucian carp. A similar trend was presented in the hepatopancreas under Cu exposure. Dietary SDE supplementation enhanced the activities of CAT, superoxide dismutase (SOD), glutathione peroxidase (GPx), lactate dehydrogenase, glutamate-oxaloacetate transaminase, and glutamate-pyruvate transaminase in the muscle of crucian carp under trichlorfon exposure. The optimum dietary SDE supplementation levels were 4.07, 4.33, and 3.95 g kg(-1) diet based on the recovery rate of weight gain (RWG), feed intake (FI), and inhibitory rate of rollover (IR) for crucian carp under high stocking density and Cu and trichlorfon exposure, respectively. Overall, dietary supplementation with SDE may be a useful nutritional strategy for relieving these stresses in aquatic animals.

Automated summary

High stocking density and Cu and trichlorfon exposure induce physiological stress in fish, causing detrimental effects such as inhibition of growth performance and reduced feed intake. This study investigates the protective effects of dietary supplementation with Scoparia dulcis extract on fish under these stressors. The results suggest that dietary supplementation with SDE may be a useful nutritional strategy for relieving the stresses induced by high stocking density and Cu and trichlorfon exposure in fish.