Effect of Chitosan on Ruminal Fermentation and Microbial Communities, Methane Emissions, and Productive Performance of Dairy Cattle

Simple Summary Expanding our understanding of the changes induced by chitosan supplementation on the rumen microbiome and its complexity is not only a key factor in finding strategies to decrease CH4 production from ruminants but also in enhancing our knowledge on the mechanisms of action of chitosan in the rumen. Therefore, this study aimed to expand the knowledge about the activity and mode of action of chitosan on methanogenesis and rumen microbial taxonomy. This is the first study, to our knowledge, accounting for the effects of chitosan on ruminal microorganisms on in vivo conditions and using whole-metagenome sequencing. Chitosan did not affect rumen microbial diversity but induced shifts in the relative abundance of some microbial taxa. Chitosan increased relative abundance of Anaeroplasma and tended to reduce fibrolytic fungi and protozoa, resulting in a shift of ruminal fermentation towards a lower acetic to propionic ratio. However, CH4 emissions, microbial protein synthesis, and productive performance were not affected by CHI supplementation. The dose of chitosan used in this study seemed not to be large enough to have a concomitant improvement in animal performance and CH4 reduction. Therefore, future works with higher doses would be necessary to assess the potential use of this additive as methane inhibitor.Abstract This study aimed to expand the knowledge about the activity and mode of action of CHI on methanogenesis and rumen microbial populations in vivo. A total of 16 lactating dairy cows were distributed in two groups, one of them receiving 135 mg CHI/kg body weight daily. The effect on productive performance, milk composition, fermentation efficiency, methane emissions, microbial protein synthesis, and ruminal microbial communities was determined. Supplementation with CHI did not affect rumen microbial diversity but increased the relative abundance (RA) of the bacteria Anaeroplasma and decreased those of rumen ciliates and protozoa resulting in a shift towards a lower acetic to propionic ratio. However, no effect on milk yield or methane intensity was observed. In conclusion, supplementing 135 mg CHI/kg body weight increased the RA of Anaeroplasma and decreased those of rumen ciliates and protozoa, both being related to fiber degradation in the rumen in different ways and resulted in a shift of ruminal fermentation towards more propionate proportions, without affecting CH4 emissions, milk yield, or milk composition. Further research with higher doses would be necessary to assess the potential use of this additive as a methane inhibitor.

Automated summary

This study used whole-metagenome sequencing to investigate the effects of chitosan on rumen microbial taxonomy and methanogenesis. The results showed that chitosan did not affect microbial diversity but induced shifts in the relative abundance of certain microbial taxa. However, chitosan supplementation did not have any significant impact on CH4 emissions, microbial protein synthesis, and productive performance.