4.7 Article

cCPE Fusion Proteins as Molecular Probes to Detect Claudins and Tight Junction Dysregulation in Gastrointestinal Cell Lines, Tissue Explants and Patient-Derived Organoids

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Biochemistry & Molecular Biology

Claudin-4: A New Molecular Target for Epithelial Cancer Therapy

Rina Fujiwara-Tani et al.

Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Medicine, General & Internal

Zolbetuximab plus mFOLFOX6 in patients with CLDN18.2-positive, HER2-negative, untreated, locally advanced unresectable or metastatic gastric or gastro- oesophageal junction adenocarcinoma (SPOTLIGHT) : a multicentre, randomised, double-blind, phase 3 trial

Kohei Shitara et al.

Summary: Zolbetuximab in combination with mFOLFOX6 significantly prolongs progression-free survival and overall survival in patients with CLDN18.2-positive, HER2-negative, locally advanced unresectable or metastatic gastric or gastro-oesophageal junction adenocarcinoma, with good safety profile.

LANCET (2023)

Review Medicine, General & Internal

Anti-Claudin Treatments in Gastroesophageal Adenocarcinoma: Mainstream and Upcoming Strategies

Giulia Grizzi et al.

Summary: Claudins (CLDNs) are proteins that compose tight junctions and mediate cell-cell adhesion and paracellular flux. CLDN18.2 is a promising target for the treatment of gastroesophageal adenocarcinoma (GEAC), especially in the genomically stable subgroup and diffuse histological subtype. Monoclonal antibody zolbetuximab and CAR-T cells targeting CLDN18.2 have shown potential efficacy.

JOURNAL OF CLINICAL MEDICINE (2023)

Article Biochemistry & Molecular Biology

Claudin-10b cation channels in tight junction strands: Octameric-interlocked pore barrels constitute paracellular channels with low water permeability

Santhosh Kumar Nagarajan et al.

Summary: Claudin proteins are crucial for regulating paracellular permeability in tight junctions. The architectural models of claudin-10b and claudin-15 suggest an octameric-interlocked-barrels structure, which contributes to their functional differences in ion and water permeability.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2023)

Article Nanoscience & Nanotechnology

Targeting prostate cancer with Clostridium perfringens enterotoxin functionalized nanoparticles co-encapsulating imaging cargo enhances magnetic resonance imaging specificity

Darryl T. Martin et al.

Summary: Magnetic resonance is a key imaging tool for detecting prostate cancer, but more cancer-specific tools are needed to distinguish benign from cancerous regions. Higher expression of CLDN-3 and CLDN-4 was found in higher-grade prostate cancer, leading to the development of functionalized nanoparticles with a strong binding affinity to these receptors. The use of these nanoparticles improved tumor specificity in a prostate tumor-bearing mouse model, demonstrating potential clinical applicability.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE (2022)

Review Oncology

Claudins and Gastric Cancer: An Overview

Itaru Hashimoto et al.

Summary: This review highlights the biological and clinical significance of claudin (CLDN) expression in gastric cancer (GC), with a focus on CLDN18.2 and clinical trials centered around it. Abnormal expression of CLDNs, particularly CLDN18, is observed in GC. CLDNs are potential targets for antibody drugs in GC treatment.

CANCERS (2022)

Article Biochemistry & Molecular Biology

Claudin18.2-specific CAR T cells in gastrointestinal cancers: phase 1 trial interim results

Changsong Qi et al.

Summary: The interim analysis results of a phase 1 clinical trial show that CLDN18.2-targeted CAR T cell therapy has promising efficacy and an acceptable safety profile in previously treated, CLDN18.2-positive digestive system cancer patients. Particularly, it shows significant efficacy in patients with gastric cancer.

NATURE MEDICINE (2022)

Review Cell Biology

Disruption of Claudin-Made Tight Junction Barriers by Clostridium perfringens Enterotoxin: Insights from Structural Biology

Chinemerem P. Ogbu et al.

Summary: Claudins are integral membrane proteins that play a crucial role in cell-cell interactions by forming tight junctions. The bacterium Clostridium perfringens causes gastrointestinal disorders by targeting claudins with its enterotoxin (CpE), disrupting the barrier function of the gut. Understanding the structure and function of Claudin and CpE provides insights into the mechanisms underlying the disruption of tight junctions. This review summarizes the current progress in this field and highlights the importance of structural studies in developing therapeutic strategies.
Article Medicine, General & Internal

High Expression of Claudin-4 Is Associated with Synchronous Tumors in Patients with Early Gastric Cancer

Won Shik Kim et al.

Summary: This study investigated the differential expression of CLDN-4 in early gastric cancer (EGC) and found a significant association between high CLDN-4 expression and intestinal-type EGC, open-type atrophic change, and the presence of synchronous tumors. These findings suggest that CLDN-4 may serve as an auxiliary tool for screening synchronous tumors in patients with EGC.

JOURNAL OF CLINICAL MEDICINE (2022)

Article Multidisciplinary Sciences

Claudin targeting as an effective tool for directed barrier modulation of the viable epidermis

Laura-Sophie Beier et al.

Summary: The formation of tight junction (TJ) is crucial for epidermal barrier function. Manipulation of TJ barriers in reconstructed human epidermis (RHE) was achieved through knockdown of claudin-1 and -4 or by using claudin-binding fusion proteins. The study demonstrates the critical role of claudin-1 in regulating paracellular permeability in the viable epidermis.

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (2022)

Article Physiology

Molecular mechanism of claudin-15 strand flexibility: A computational study

Shadi Fuladi et al.

Summary: This study investigates the structure and mechanism of Claudins in tight junctions using molecular dynamics simulations. The results demonstrate that Claudins regulate paracellular permeability and play a crucial role in the formation and maintenance of the epithelial barrier function. The flexibility of Claudin-15 strands is determined by the interplay of interfacial interaction networks in the lipid membranes.

JOURNAL OF GENERAL PHYSIOLOGY (2022)

Review Biochemistry & Molecular Biology

Multiscale modelling of claudin-based assemblies: A magnifying glass for novel structures of biological interfaces

Alessandro Berselli et al.

Summary: Claudins (Cldns) are transmembrane proteins that play a major role in tight junction integrity and tissue selectivity. Computational methodologies have been used to investigate the properties of Cldn and provide valuable insights for improving the characterization of Cldn and designing strategies for controlling paracellular transport.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2022)

Article Biochemistry & Molecular Biology

Multiscale modelling of claudin-based assemblies: A magnifying glass for novel structures of biological interfaces

Alessandro Berselli et al.

Summary: Claudins are transmembrane proteins that play a crucial role in tight junction integrity and tissue selectivity. They form barriers or ion-selective channels between cells in the paracellular space. Computational methodologies have helped to elucidate the properties of Claudins and could be useful for designing strategies to control the transport of drugs or molecules.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2022)

Review Physiology

Targeting desmosomal adhesion and signalling for intestinal barrier stabilization in inflammatory bowel diseases-Lessons from experimental models and patients

Nicolas Schlegel et al.

Summary: Inflammatory bowel diseases such as Crohn's disease and Ulcerative colitis have a complex pathogenesis, with impaired intestinal barrier function being a key feature closely associated with clinical symptoms. Dsg2, a cadherin-type adhesion molecule of desmosomes, plays a distinct role in IBD pathogenesis by regulating intestinal barrier properties and is a promising target for preventing loss of barrier function in IBD patients.

ACTA PHYSIOLOGICA (2021)

Article Biochemistry & Molecular Biology

Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention

Suhayla Alnajjar et al.

Summary: This study utilized recombinant C-CPE conjugated to gold nanoparticles for targeting prostate adenocarcinoma and transitional cell carcinoma cells in vitro, demonstrating efficient binding and potential immune activation effects. Transcriptome analysis indicated that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. The combination of GNOME-LP and C-CPE-AuNPs treatment was effective in reducing tumor cell viability to less than 10% depending on cell line, providing a basis for future xenograft studies in animal models.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells

Carlos Ayala-Torres et al.

Summary: Water transport in epithelia is regulated by tight junction proteins, with modulation of these proteins affecting ion and macromolecule permeability in tight and intermediate-tight epithelial cell lines. However, transepithelial water permeability is only affected in tight cell lines.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Chemistry, Multidisciplinary

Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood-brain barrier permeability for drug delivery to the brain in a non-human primate

Keisuke Tachibana et al.

Summary: The study suggests that CLDN-5 may be a potential target for enhancing drug delivery to the brain, but the therapeutic window of the anti-CLDN-5 mAb may be narrow.

JOURNAL OF CONTROLLED RELEASE (2021)

Editorial Material Cell Biology

The 2019 WHO classification of tumours of the digestive system

Iris D. Nagtegaal et al.

HISTOPATHOLOGY (2020)

Review Biochemistry & Molecular Biology

Molecular architecture and assembly of the tight junction backbone

Joerg Piontek et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Article Gastroenterology & Hepatology

Increased Intestinal Permeability Is Associated With Later Development of Crohn 's Disease

Williams Turpin et al.

GASTROENTEROLOGY (2020)

Review Pharmacology & Pharmacy

Tight Junction Modulating Bioprobes for Drug Delivery System to the Brain: A Review

Keisuke Tachibana et al.

PHARMACEUTICS (2020)

Article Gastroenterology & Hepatology

Human gastric cancer modelling using organoids

Therese Seidlitz et al.

Article Biochemistry & Molecular Biology

Use of Modified Clostridium perfringens Enterotoxin Fragments for Claudin Targeting in Liver and Skin Cells

Laura-Sophie Beier et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

Article Cell Biology

Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity

Tetsuhisa Otani et al.

JOURNAL OF CELL BIOLOGY (2019)

Review Pharmacology & Pharmacy

Anti-Claudin Antibodies as a Concept for Development of Claudin-Directed Drugs

Yosuke Hashimoto et al.

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS (2019)

Article Gastroenterology & Hepatology

An Organoid-Based Preclinical Model of Human Gastric Cancer

Nina G. Steele et al.

CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY (2019)

Review Physiology

Active and passive involvement of claudins in the pathophysiology of intestinal inflammatory diseases

Christian Barmeyer et al.

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2017)

Article Pharmacology & Pharmacy

Claudin-5-Binders Enhance Permeation of Solutes across the Blood-Brain Barrier in a Mammalian Model

Yosuke Hashimoto et al.

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS (2017)

Article Multidisciplinary Sciences

A cCPE-based xenon biosensor for magnetic resonance imaging of claudin-expressing cells

Anna Piontek et al.

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (2017)

Review Biochemistry & Molecular Biology

Modeling Development and Disease with Organoids

Hans Clevers

Article Radiology, Nuclear Medicine & Medical Imaging

Claudin-4 SPECT Imaging Allows Detection of Aplastic Lesions in a Mouse Model of Breast Cancer

Michael Mosley et al.

JOURNAL OF NUCLEAR MEDICINE (2015)

Article Multidisciplinary Sciences

Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin

Yasunori Saitoh et al.

SCIENCE (2015)

Review Cell Biology

Claudin-related intestinal diseases

Christian Barmeyer et al.

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Directed structural modification of Clostridium perfringens enterotoxin to enhance binding to claudin-5

Jonas Protze et al.

CELLULAR AND MOLECULAR LIFE SCIENCES (2015)

Article Gastroenterology & Hepatology

Claudin-4-targeted optical imaging detects pancreatic cancer and its precursor lesions

Albrecht Neesse et al.

Review Biochemistry & Molecular Biology

Emerging Roles of Claudins in Human Cancer

Mi Jeong Kwon

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2013)

Article Biochemical Research Methods

In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture

Hiroyuki Miyoshi et al.

NATURE PROTOCOLS (2013)

Article Engineering, Biomedical

Creation and biochemical analysis of a broad-specific claudin binder

Azusa Takahashi et al.

BIOMATERIALS (2012)

Article Biochemical Research Methods

Fiji: an open-source platform for biological-image analysis

Johannes Schindelin et al.

NATURE METHODS (2012)

Article Biochemistry & Molecular Biology

Predicted expansion of the claudin multigene family

Katsuhiko Mineta et al.

FEBS LETTERS (2011)

Article Medicine, Research & Experimental

Claudin-4 overexpression is associated with epigenetic derepression in gastric carcinoma

Mi Jeong Kwon et al.

LABORATORY INVESTIGATION (2011)

Article Multidisciplinary Sciences

Claudin-7 Is Frequently Overexpressed in Ovarian Cancer and Promotes Invasion

Neetu Dahiya et al.

PLOS ONE (2011)

Article Biochemistry & Molecular Biology

Effects of Clostridium perfringens enterotoxin via claudin-4 on normal human pancreatic duct epithelial cells and cancer cells

Hiroshi Yamaguchi et al.

CELLULAR & MOLECULAR BIOLOGY LETTERS (2011)

Article Biochemistry & Molecular Biology

A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin

Doerte Lohrberg et al.

BMC MOLECULAR BIOLOGY (2009)

Article Biochemistry & Molecular Biology

Molecular Determinants of the Interaction between Clostridium perfringens Enterotoxin Fragments and Claudin-3

Lars Winkler et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Review Biochemistry & Molecular Biology

Adherens and tight junctions: Structure, function and connections to the actin cytoskeleton

Andrea Hartsock et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2008)

Article Gastroenterology & Hepatology

Changes in the expression of claudins in active ulcerative colitis

Tadayuki Oshima et al.

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2008)

Article Medicine, Research & Experimental

Claudin-1 and claudin-2 expression is elevated in inflammatory bowel disease and may contribute to early neoplastic transformation

Christopher R. Weber et al.

LABORATORY INVESTIGATION (2008)

Article Medicine, Research & Experimental

Inflammatory processes have differential effects on claudins 2, 3 and 4 in colonic epithelial cells

S Prasad et al.

LABORATORY INVESTIGATION (2005)

Article Medicine, Research & Experimental

Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer

P Dhawan et al.

JOURNAL OF CLINICAL INVESTIGATION (2005)

Article Gastroenterology & Hepatology

Early aldosterone effect in distal colon by transcriptional regulation of ENaC subunits

HJ Epple et al.

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY (2000)