☆ 4.7 Article

CRISPR/Cas9 systems: delivery technologies and biomedical applications

Asian Journal of Pharmaceutical Sciences (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Article Biochemistry & Molecular Biology

Development of a CRISPRi Human Retinal Pigmented Epithelium Model for Functional Study of Age-Related Macular Degeneration Genes

Jiang-Hui Wang et al.

Summary: Age-related macular degeneration (AMD) is a blinding disease characterized by dysfunction of the retinal pigmented epithelium (RPE). This study developed a human RPE model using CRISPRi technology, providing a useful tool for functional studies of AMD-associated genes.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

添加到收藏夹

Article Chemistry, Physical

Mitoxantrone and Mitoxantrone-Loaded Iron Oxide Nanoparticles Induce Cell Death in Human Pancreatic Ductal Adenocarcinoma Cell Spheroids

Jonas Dinter et al.

Summary: Pancreatic ductal adenocarcinoma is a difficult-to-treat cancer with poor long-term survival rates. In this study, a 3D cell culture model of human pancreatic ductal adenocarcinoma was used to investigate the potential use of superparamagnetic iron oxide nanoparticles (SPIONs) as a drug delivery system for the chemotherapeutic agent mitoxantrone (MTO). The results showed that MTO-loaded SPIONs induced cell death in tumor spheroids, with increased uptake in spheroids with a SMAD4 mutation. This suggests that MTO-loaded SPIONs could be a promising approach for treating pancreatic ductal adenocarcinomas.

MATERIALS (2023)

添加到收藏夹

Article Oncology

Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma

Agustin Sgro et al.

Summary: Liver-targeted delivery of CRISPRa systems enables specific treatment of aggressive hepatocellular carcinoma (HCC) by reactivating tumor suppressor genes (TSGs) and inhibiting multiple aspects of HCC pathogenesis. This study identifies 12 putative TSGs in HCC and demonstrates the utility of a CRISPRa toolbox for personalized treatment.

CLINICAL EPIGENETICS (2023)

添加到收藏夹

Article Medicine, Research & Experimental

In vivo editing of the pan-endothelium by immunity evading simian adenoviral vector

Reka Lorincz et al.

Summary: Biological applications of the CRISPR/Cas9 nuclease system have had a significant impact on gene therapy strategies. However, challenges such as vector immunity and off-target editing still need to be addressed. In this study, we have developed a modified SAd36. MBP vector through adenovirus engineering, enabling selective and effective gene editing of target cells in mice spleen, brain, and kidney.

BIOMEDICINE & PHARMACOTHERAPY (2023)

添加到收藏夹

Article Biology

Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo

Zeming Zhang et al.

Summary: In this study, an integrated CRISPR/Cas9 system was established for gene knock-out and knock-in at a specific site in medaka. The system is simple, cost-effective, and efficient. The results showed that pCas9-mU6sgRNA driven by endogenous U6 promoter achieved high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. This study provides insights into other fish genome editing and promotes gene functional analysis.

BIOLOGY-BASEL (2023)

添加到收藏夹

Article Chemistry, Inorganic & Nuclear

Delivery of dCas9 Activator System Using Magnetic Nanoparticles Technology as a Vector Delivery Method for Human Skin Fibroblast

Mahdi Mohammadi Ghanbarlou et al.

Summary: The overexpression of stem cell-related genes can promote stem cell self-renewal and increase self-renewal of cancer cells with stem cell-like properties. CRISPR/Cas9 protein fused with transactivation domains can activate gene expression in human cells. We used superparamagnetic iron oxide nanoparticles coated with polyethylenimine to enhance the delivery of CRISPR/Cas9 constructs into human foreskin fibroblasts.

MAGNETOCHEMISTRY (2023)

添加到收藏夹

Article Oncology

Evaluation of Radiation Sensitivity Differences in Mouse Liver Tumor Organoids Using CRISPR/Cas9-Mediated Gene Mutation

Wan Jeon et al.

Summary: A radiation response assessment system was developed using CRISPR/Cas9 and organoids to evaluate mouse tumors with mutant target genes. The combination of Tp53 and Pten double mutation with Nf2 mutation increased tumor resistance to radiation.

TECHNOLOGY IN CANCER RESEARCH & TREATMENT (2023)

添加到收藏夹

Article Chemistry, Multidisciplinary

A Proton-Activatable DNA-Based Nanosystem Enables Co-Delivery of CRISPR/Cas9 and DNAzyme for Combined Gene Therapy

Feng Li et al.

Summary: This study reports a proton-activatable DNA-based nanosystem that enables co-delivery of Cas9/sgRNA and DNAzyme for combined gene therapy of breast cancer.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

添加到收藏夹

Article Engineering, Biomedical

Knock-In of a Large Reporter Gene via the High-Throughput Microinjection of the CRISPR/Cas9 System

Shuxun Chen et al.

Summary: Microinjection of CRISPR/Cas9 system enables efficient gene knock-in by directly injecting CRISPR components and donor templates into single cells.

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING (2022)

添加到收藏夹

Review Pharmacology & Pharmacy

Delivery strategies for CRISPR/Cas genome editing tool for retinal dystrophies: challenges and opportunities

Aayushi Lohia et al.

Summary: CRISPR/Cas, as an efficient and precise tool, has shown potential in genetic editing for retinal dystrophies. The reversal of gene mutations using CRISPR/Cas9 could be beneficial for the treatment of these conditions. However, the delivery of CRISPR components to the posterior part of the eye remains a major concern.

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Allele-Specific Knockdown of Mutant Huntingtin Protein via Editing at Coding Region Single Nucleotide Polymorphism Heterozygosities

Sarah R. Oikemus et al.

Summary: Huntington's disease is a neurodegenerative disease caused by a trinucleotide repeat expansion in the huntingtin gene. Inactivation of the mutant allele using CRISPR-Cas9 gene editing offers a potential therapeutic approach, and targeting a protein coding sequence containing a single nucleotide polymorphism allows for allele-specific inactivation. This study successfully demonstrated allele-selective reduction of mutant huntingtin protein in a mouse model of the disease.

HUMAN GENE THERAPY (2022)

添加到收藏夹

Article Nanoscience & Nanotechnology

Enhancing CRISPR/Cas gene editing through modulating cellular mechanical properties for cancer therapy

Di Zhang et al.

Summary: Modulating the mechanical properties of tumor cells enhances gene editing and inhibits tumor growth by using dendrimer lipid nanoparticles for PD-L1 gene editing and knocking out FAK.

NATURE NANOTECHNOLOGY (2022)

添加到收藏夹

Article Biophysics

Aptamer based probes for living cell intracellular molecules detection

Xiaoqin Liu et al.

Summary: This article summarizes fluorescence detection methods based on aptamers for intracellular molecular detection. By combining different nanostructures, these biosensors can enter living cells for analysis and enable in situ monitoring. The principles, limit of detection, advantages, and disadvantages of different platforms for intracellular molecular fluorescence response are reviewed, and the current challenges and future developments are discussed.

BIOSENSORS & BIOELECTRONICS (2022)

添加到收藏夹

Review Pharmacology & Pharmacy

Progress of delivery methods for CRISPR-Cas9

Wu Yang et al.

Summary: Gene therapy using the CRISPR/Cas9 system has great potential in correcting human diseases and defects. However, delivering the CRISPR-Cas9 to target cells remains a challenge. Researchers have developed various technologies to deliver CRISPR-Cas9 for in vitro and in vivo gene editing.

EXPERT OPINION ON DRUG DELIVERY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Full-Length Dystrophin Restoration via Targeted Exon Addition in DMD-Patient Specific iPSCs and Cardiomyocytes

Rou Xiao et al.

Summary: This study demonstrated the successful restoration of full-length dystrophin in iPSCs and iCMs through targeted exon addition using CRISPR/Cas9 technology, showing potential clinical prospects for DMD gene therapy.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

添加到收藏夹

Article Immunology

Naive Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

Petra Pfenninger et al.

Summary: CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells, including CD8(+) T cells. The optimized nucleofection-based CRISPR/Cas9 genetic engineering method preserves the in vivo immune responses of naive CD8(+) T cells, but slightly impairs the expansion and survival of in vitro-activated CD8(+) T cells.

FRONTIERS IN IMMUNOLOGY (2022)

添加到收藏夹

Article Pharmacology & Pharmacy

Nanodevices for the Efficient Codelivery of CRISPR-Cas9 Editing Machinery and an Entrapped Cargo: A Proposal for Dual Anti-Inflammatory Therapy

Alba Garcia-Fernandez et al.

Summary: This article reports the development of nanoparticles capable of delivering CRISPR-Cas9 gene-editing machinery and releasing drugs for one-shot treatments. The nanoparticles show promise in treating inflammatory diseases by simultaneously editing the key protein involved in inflammatory cell death and delivering an anti-inflammatory drug. The study demonstrates the potential of these nanoparticles as a versatile platform for gene-editing and drug therapy.

PHARMACEUTICS (2022)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Genome edited wheat- current advances for the second green revolution

Muhammad Jawad Akbar Awan et al.

Summary: Common wheat, with its complex genome, poses challenges for crop improvement. However, advancements in genomics technologies, such as the CRISPR-Cas9 system, along with tissue culturing and speed breeding, offer promising opportunities to enhance wheat yield and disease resistance.

BIOTECHNOLOGY ADVANCES (2022)

添加到收藏夹

Article Chemistry, Applied

A novel dual-targeting delivery system for specific delivery of CRISPR/Cas9 using hyaluronic acid, chitosan and AS1411

Zahra Khademi et al.

Summary: A facile method was developed for specific delivery of CRISPR/Cas9 into the nuclei of target cells and reducing off-target effects. The use of a multifunctional delivery vector composed of hyaluronic acid and AS1411 aptamer on the surface of nanoparticles enabled successful internalization of CRISPR/Cas9 into target cells while avoiding non-target cells. In vivo studies demonstrated efficient delivery of CRISPR/Cas9 into tumors with minimal distribution in other organs, providing a promising avenue for specific gene editing therapeutics.

CARBOHYDRATE POLYMERS (2022)

添加到收藏夹

Review Pharmacology & Pharmacy

CRISPR-Cas9 based non-viral approaches in nanoparticle elicited therapeutic delivery

Vinitha Rani et al.

Summary: The CRISPR-Cas9 genome editing technology has been gaining attention in basic research and is considered a powerful tool for improving therapeutic strategies for various genetic disorders. The development of non-viral drug delivery systems has made nanocarriers important in delivering CRISPR-Cas9. This review outlines recent developments in using non-viral vectors as potential carriers for genome editing and discusses the advantages and limitations of non-viral-based CRISPR-Cas9 editing and how to overcome these limitations.

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022)

添加到收藏夹

Article Pharmacology & Pharmacy

Lipid nanoparticle-mediated CRISPR/Cas9 gene editing and metabolic engineering for anticancer immunotherapy

Hyemin Ju et al.

Summary: This study developed a cationic lipid nanoparticle formulation for LDHA gene editing and highlighted the potential of activating immune responses in the tumor microenvironment.

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022)

添加到收藏夹

Article Pharmacology & Pharmacy

A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing

Lingmin Zhang et al.

Summary: A novel gene vector ZEBRA was developed for efficient delivery of the CRISPR/Cas9 system, showing cell line dependency with approximately 10-fold higher transduction efficiency in CD44-positive cancer cell lines compared to CD44-negative ones.

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022)

添加到收藏夹

Article Gastroenterology & Hepatology

CRISPR-targeted genome editing of human induced pluripotent stem cell-derived hepatocytes for the treatment of Wilson's disease

Rui Wei et al.

Summary: The study demonstrated that gene-corrected patient-specific iPSC-derived iHeps can rescue the in vitro and in vivo disease phenotypes of WD, providing potential for in vivo therapy of WD as well as other inherited liver disorders.

JHEP REPORTS (2022)

添加到收藏夹

Review Chemistry, Multidisciplinary

Spatiotemporal Delivery of CRISPR/Cas9 Genome Editing Machinery Using Stimuli-Responsive Vehicles

Weiqi Cai et al.

Summary: Recent innovations in genome editing have led to precise manipulation of genetic information in mammals using stimuli-responsive nanoparticles for the delivery and release of CRISPR/Cas9 genome editing machinery. These approaches can utilize biological signals or external signals to achieve improved genome editing outcomes.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

添加到收藏夹

Article Oncology

Targeting RLIP with CRISPR/Cas9 controls tumor growth

Jyotsana Singhal et al.

Summary: This study successfully targeted the RLIP gene in breast cancer cells and knocked down its expression using CRISPR/Cas9 technology, demonstrating its potential to inhibit the proliferation and survival of BC cells in vitro and in vivo.

CARCINOGENESIS (2021)

添加到收藏夹

Article Biology

Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras(G12D) in pancreatic cancer

Kathleen M. McAndrews et al.

Summary: CRISPR/Cas9 technology shows promise for gene editing, and exosomes engineered as non-viral delivery systems can successfully deliver CRISPR/Cas9 for targeted gene editing in cancer cells, demonstrating potential therapeutic applications.

LIFE SCIENCE ALLIANCE (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence

Kesavan Babu et al.

Summary: CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection against intruding genomic materials. This study demonstrates coordinated actions between HNH and RuvC in DNA linearization, modulated by BH substitutions and DNA mismatches, affecting the use of parallel sequential pathways. The results suggest that BH interactions with RNA/DNA enable target DNA discrimination through differential use of pathways driven by HNH/RuvC coordination.

BIOCHEMISTRY (2021)

添加到收藏夹

Article Engineering, Biomedical

Transcriptional activation with Cas9 activator nanocomplexes rescues Alzheimer's disease pathology

Hanseul Park et al.

Summary: The development of a Cas9 activator nanocomplex that efficiently activates endogenous genes in the brain in vivo suggests its potential in novel therapeutic applications. Activating the Adam10 gene in the mouse brain using the Cas9 activator nanocomplex improved cognitive deficits in an Alzheimer's disease model, demonstrating therapeutic potential for a wide range of neurological diseases.

BIOMATERIALS (2021)

添加到收藏夹

Article Chemistry, Physical

Delivery of CRISPR/Cas9 Plasmids by Cationic Gold Nanorods: Impact of the Aspect Ratio on Genome Editing and Treatment of Hepatic Fibrosis

Yuxuan Chen et al.

Summary: This study demonstrates that cationic polymer-coated gold nanorods can efficiently mediate the delivery of CRISPR/Cas9 plasmids into cells, enabling genome editing and transcriptional activation. The unique nanomaterial structure contributes to genome-editing activity and provides a new method for the efficient delivery of CRISPR/Cas9 plasmids, showing potential therapeutic benefits in a mouse model.

CHEMISTRY OF MATERIALS (2021)

添加到收藏夹

Article Nanoscience & Nanotechnology

Gene Therapy for Drug-Resistant Glioblastoma via Lipid-Polymer Hybrid Nanoparticles Combined with Focused Ultrasound

Qiang Yang et al.

Summary: In this study, LPHNs-cRGD were developed for targeted delivery of the CRISPR/Cas9 system, combined with FUS-MBs to open the BBB. The delivery system showed potential as an efficient targeted gene delivery option for treating glioblastoma.

INTERNATIONAL JOURNAL OF NANOMEDICINE (2021)

添加到收藏夹

Article Cell Biology

A new mouse model of Ehlers-Danlos syndrome generated using CRISPR/Cas9-mediated genomic editing

Yuko Nitahara-Kasahara et al.

Summary: Researchers generated mouse models for musculocontractural Ehlers-Danlos syndrome (mcEDS) through CRISPR/Cas9 genome editing, revealing pathophysiological features such as growth impairment, skin fragility, and muscle-related phenotypes caused by depletion of dermatan sulfate (DS). This study provides insights into the pathophysiology of mcEDS and may contribute to the development of novel treatment strategies.

DISEASE MODELS & MECHANISMS (2021)

添加到收藏夹

Article Biochemical Research Methods

AAV-mediated in vivo genome editing in vascular endothelial cells

Wenyi Wu et al.

Summary: This method delivers CRISPR-Cas9 into vascular endothelial cells using AAVs, with SpCas9 expression driven by a specific endothelial promoter to restrict the foreign enzyme in these cells. By editing VEGFR2 in retinal vascular endothelial cells in a mouse model, this simplified protocol can potentially be expanded to other research on editing endothelial genome in vivo.

METHODS (2021)

添加到收藏夹

Article Oncology

CRISPR/Cas correction of muscular dystrophies

Yu Zhang et al.

Summary: Muscular dystrophies are a group of neuromuscular disorders with genetic causes that lead to muscle loss and degeneration. The CRISPR/Cas system offers a new path for treatment, potentially correcting genetic mutations permanently and benefiting skeletal muscle due to its post-mitotic and multinucleated features. However, challenges remain for translating CRISPR/Cas genome editing into a viable therapy for muscular dystrophies.

EXPERIMENTAL CELL RESEARCH (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Design Strategy for the EPR Tumor-Targeting of 1,2-Bis(sulfonyl)-1-alkylhydrazines

Philip G. Penketh et al.

Summary: The design strategy involves using the EPR effect and carefully engineered rate constants to selectively target tumor tissue with short-lived cytotoxic moieties. The macromolecular prodrugs have a long half-life and release the cytotoxic warhead only after tumor sequestration, allowing for localized cytotoxic stress within the tumor target site. Abnormal tumor cell membrane pH gradients also enhance the selectivity of the prodrugs.

MOLECULES (2021)

添加到收藏夹

Article Medicine, General & Internal

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia

H. Frangoul et al.

Summary: Research utilizing CRISPR-Cas9 technology for gene editing in patients with TDT and SCD showed high levels of edited alleles in bone marrow and blood, leading to partial or complete elimination of symptoms.

NEW ENGLAND JOURNAL OF MEDICINE (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Engineering NK-92 Cell by Upregulating CXCR2 and IL-2 Via CRISPR-Cas9 Improves Its Antitumor Effects as Cellular Immunotherapy for Human Colon Cancer

Lanlan Gao et al.

Summary: By upregulating the expression of CXCR2 and IL-2 on NK-92 cells using CRISPR-Cas9 gene-editing technology, the NK-92 cells were able to increasingly transfer into tumor sites, exhibit stronger cell-killing and proliferation activity, and show enhanced inhibitory effects on human colon cancer growth. This gene-editing modification of NK cells is expected to become a novel and promising strategy for tumor treatment.

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2021)

添加到收藏夹

Article Oncology

CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer

Ailin Chen et al.

Summary: The study identified Haspin as a synthetic lethal target, and demonstrated that the combination of Haspin inhibitor CHR-6494 and MLN8237 effectively reduces breast cancer cell viability and inhibits tumor growth.

CANCER COMMUNICATIONS (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

A Duplex CRISPR-Cas9 Ribonucleoprotein Nanomedicine for Colorectal Cancer Gene Therapy

Tao Wan et al.

Summary: This study developed a CRISPR-Cas9-based genome-editing nanomedicine that targets both APC and KRAS mutations in human colorectal cancer. Systemic administration of Cas9 RNP enabled by HAPD significantly inhibited tumor growth in mouse models and prevented CRC-induced liver and lung metastasis. This duplex genome-editing system provides a promising gene therapy strategy for human CRC and potentially other cancers with similar genetic mutations.

NANO LETTERS (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition

Yuichi Suzuki et al.

Summary: This study presents a lipid nanoparticle (LNP)-based CRISPR/Cas ribonucleoprotein (RNP) delivery nanoplatform synthesized using a clinically relevant mixer-equipped microfluidic device, which avoids DNA cleavage activity and Cas enzyme aggregation. The optimized formulation, identified through 2 steps of design of experiments, showed excellent gene disruption and base substitution efficiencies without apparent cytotoxicity. The addition of negative charges to the RNPs by complexing single-stranded oligonucleotide (ssON) significantly enhanced the delivery of both Cas9 and Cpf1 RNPs.

JOURNAL OF CONTROLLED RELEASE (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3

Min Qiu et al.

Summary: Loss-of-function mutations in Angiopoietin-like 3 (Angptl3) are linked to reduced blood lipid levels, making it a potential therapeutic target for lipid metabolism disorders. A lipid nanoparticle delivery system successfully edited Angptl3 gene in vivo, leading to significant reductions in lipid levels without off-target effects or toxicity, highlighting the potential of this platform for safe and effective gene editing therapies.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice

Eriya Kenjo et al.

Summary: The study demonstrates that LNP delivery of CRISPR-Cas9 enables restored dystrophin expression in multiple muscle groups, providing a promising approach for treating muscular dystrophy.

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Cas9-specific immune responses compromise local and systemic AAV CRISPR therapy in multiple dystrophic canine models

Chady H. Hakim et al.

Summary: The study investigates the immune responses induced by AAV-CRISPR therapy in canine models of DMD, indicating that the Cas9-specific T cell response may pose a critical barrier to treatment.

NATURE COMMUNICATIONS (2021)

添加到收藏夹

Article Medicine, Research & Experimental

In vivo PCSK9 gene editing using an all-in-one self-cleavage AAV-CRISPR system

Qian Li et al.

Summary: This study engineered an all-in-one self-cleavage AAV-CRISPR-Cas9 system to restrict the expression of Cas9 nuclease, demonstrating a reduction in Cas9 protein expression and off-target activity while maintaining on-target editing efficacy in vivo. The inclusion of this self-cleavage system could enhance the safety profile of AAV-delivered genome-editing nucleases and facilitate their clinical translation.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2021)

添加到收藏夹

Article Medicine, Research & Experimental

Cas9 protein delivery non-integrating lentiviral vectors for gene correction in sickle cell disease

Naoya Uchida et al.

Summary: The development of a non-integrating lentiviral system encoding Cas9 protein, guide RNA, and donor DNA allows for efficient one-time correction of hereditary diseases, improving prospects for genome editing.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2021)

添加到收藏夹

Article Genetics & Heredity

Self-Cutting and Integrating CRISPR Plasmids Enable Targeted Genomic Integration of Genetic Payloads for Rapid Cell Engineering

Darin Bloemberg et al.

Summary: The study introduces self-cutting and integrating CRISPR-Cas9 plasmids (SCIPs) as gene editing tools, demonstrating high efficiency in gene editing by integrating at CRISPR-guided locations. After stable integration in cells, gene fragments can be precisely inserted to create cell lines with specific functions.

CRISPR JOURNAL (2021)

添加到收藏夹

Article Engineering, Biomedical

Systemic delivery of CRISPR/Cas9 to hepatic tumors for cancer treatment using altered tropism of lentiviral vector

Sungjin Lee et al.

Summary: The study demonstrated successful systemic delivery of CRISPR/Cas9 to Huh7 tumors using lentiviral vectors pseudotyped with hepatitis C virus glycoproteins, effectively inhibiting tumor growth. E1E2-pseudotyping proved to be superior for cancer therapy compared to vesicular stomatitis virus-pseudotyping due to stability, low immune response, and cell-specific targeting.

BIOMATERIALS (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

CRISPR-Cas9-Mediated TIM3 Knockout in Human Natural Killer Cells Enhances Growth Inhibitory Effects on Human Glioma Cells

Takayuki Morimoto et al.

Summary: Studies have shown that enhancing NK cell-mediated cytotoxicity on GBM cells through TIM3 knockout using CRISPR-Cas9 technology may be a promising immunotherapeutic alternative.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

添加到收藏夹

Review Genetics & Heredity

New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer

Munazza Ahmed et al.

Summary: Breast cancer is a prevalent and heterogeneous disease with complex pathogenesis, often treated by alleviating symptoms rather than targeting mutations. CRISPR/Cas9 is a groundbreaking gene-editing tool that offers a promising new approach for the treatment of breast cancer, surpassing previous methods in terms of simplicity, efficiency, and affordability.

GENES (2021)

添加到收藏夹

Article Oncology

CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines

Alessio Biagioni et al.

Summary: Loss of uPAR in melanoma and colon cancer results in cells acquiring stem-like characteristics and completely inhibits tumorigenesis. Reintroduction of the 3'UTR of PLAUR gene restores wild-type status, suggesting this region may act as a molecular sponge.

FRONTIERS IN ONCOLOGY (2021)

添加到收藏夹

Article Cell Biology

Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing

Xingang Yao et al.

Summary: A mechanism to actively enrich RNPs into EVs using RNA aptamer and aptamer-binding protein interactions has been described, resulting in efficient genome editing and transient expression. This system allows for multiplex genome editing targeting multiple loci and can deliver RNPs active in vivo. The data demonstrate that this approach could enhance genome editing efficiency and safety.

JOURNAL OF EXTRACELLULAR VESICLES (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Hypoxia-Responsive Gene Editing to Reduce Tumor Thermal Tolerance for Mild-Photothermal Therapy

Xueqing Li et al.

Summary: This study develops a hypoxia-responsive nanocomposite for mild-photothermal therapy through gene editing in tumors. The technology has the potential to enhance tumor ablation efficiency and reduce damage to surrounding healthy tissues.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

添加到收藏夹

Article Chemistry, Physical

Obstacles and opportunities in a forward vision for cancer nanomedicine

Irene de Lazaro et al.

Summary: This text discusses the biological barriers that have hindered the clinical translation of cancer nanomedicines, and elaborates on new directions for the field that capitalize on a deeper understanding of the nano-bio interface.

NATURE MATERIALS (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

A cleavage-based surrogate reporter for the evaluation of CRISPR-Cas9 cleavage efficiency

Soo Bin Jung et al.

Summary: CRISPR-Cas9 is a powerful tool for genome engineering, and a LacI-reporter system was developed in the study to evaluate gRNA cleavage efficiency by quantitating luciferase activity or EGFP expression. The study found a strong positive correlation between the efficiency of gRNAs measured using this system and mutation frequency measured using surveyor and deep sequencing, with validation in human liver organoids. The LacI-reporter system provides a useful tool for selecting efficient gRNAs for genome editing.

NUCLEIC ACIDS RESEARCH (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Supramolecular Nanosubstrate-Mediated Delivery for CRISPR/Cas9 Gene Disruption and Deletion

Qian Ban et al.

Summary: CRISPR/Cas9 technology offers a versatile solution for treating genetic diseases through efficient and precise gene editing. A nonviral delivery strategy involving encapsulating Cas9·sgRNA ribonucleoprotein complexes into supramolecular nanoparticles has shown promise in targeted cell delivery, demonstrating efficient gene disruption in various cell types.

SMALL (2021)

添加到收藏夹

Article Biotechnology & Applied Microbiology

CRISPR/Cas9 nanoeditor of double knockout large fragments of E6 and E7 oncogenes for reversing drugs resistance in cervical cancer

Xianhuang Li et al.

Summary: A nanoeditor was designed to knockout two key oncogenes associated with drug resistance in cervical cancer, effectively reactivating signaling pathways that inhibit tumor cell growth. When combined with chemotherapy drugs, the nano system successfully inhibits drug tolerance in cancer cells and improves therapeutic outcomes.

JOURNAL OF NANOBIOTECHNOLOGY (2021)

添加到收藏夹

Review Chemistry, Physical

Improving the Delivery of Drugs and Nucleic Acids to T Cells Using Nanotechnology

Jenny Lou et al.

Summary: T cells play important roles in antitumor immunity, but issues such as low efficiency and non-selective drug delivery to T cells hinder the benefits of immunotherapies for some patients. Nanotechnology offers potential solutions for improving drug delivery to T cells.

SMALL STRUCTURES (2021)

添加到收藏夹

Review Materials Science, Biomaterials

Harnessing lipid nanoparticles for efficient CRISPR delivery

Jingyue Yan et al.

Summary: The CRISPR-Cas system has revolutionized biomedical research with its simple and flexible genome editing method. Recent advances in novel lipid nanomaterials for CRISPR delivery show potential for therapeutics in cancers, genetic disorders, and infectious diseases, despite challenges in specific and effective in vivo delivery of CRISPR.

BIOMATERIALS SCIENCE (2021)

添加到收藏夹

Article Pharmacology & Pharmacy

Effective Delivery of the CRISPR/Cas9 System Enabled by Functionalized Mesoporous Silica Nanoparticles for GFP-Tagged Paxillin Knock-In

Xiaoyu Xu et al.

Summary: This study achieved direct intracellular delivery of CRISPR/Cas9 plasmids for homology-directed repair using functionalized mesoporous silica nanoparticles. The polymeric coating of PDDA on plasmid-loaded MSNs enhanced cellular internalization and endosomal escape, leading to successful gene editing.

ADVANCED THERAPEUTICS (2021)

添加到收藏夹

Article Education & Educational Research

Content knowledge and social factors influence student moral reasoning about CRISPR/Cas9 in humans

Katie M. Seiter et al.

Summary: Consideration of socioscientific issues helps develop moral and sociocultural perspectives, enriching the understanding of science. In examining students' moral reasoning on using CRISPR/Cas9 technology for nonmedical enhancement, it was found that they focused on moral controversies related to eugenics, equity, diversity, risk, and natural authority. Different levels of content knowledge, gender, socioeconomic status, and first-generation status influenced students' moral considerations, indicating complex relationships between these factors.

JOURNAL OF RESEARCH IN SCIENCE TEACHING (2021)

添加到收藏夹

Review Medicine, Research & Experimental

Engineering exosomes for targeted drug delivery

Yujie Liang et al.

Summary: Exosomes can be engineered through genetic and chemical methods for targeted drug delivery, increasing the local concentration of therapeutics and minimizing side effects.

THERANOSTICS (2021)

添加到收藏夹

Article Materials Science, Biomaterials

Coassembly of nucleus-targeting gold nanoclusters with CRISPR/Cas9 for simultaneous bioimaging and therapeutic genome editing

Yu Tao et al.

Summary: The study introduces protamine-capped gold nanoclusters as an effective CRISPR/Cas9 delivery tool for achieving successful gene editing and cancer therapy.

JOURNAL OF MATERIALS CHEMISTRY B (2021)

添加到收藏夹

Article Chemistry, Physical

Chain-shattering Pt(IV)-backboned polymeric nanoplatform for efficient CRISPR/Cas9 gene editing to enhance synergistic cancer therapy

Qingfei Zhang et al.

Summary: A chain-shattering Pt(IV)-backboned polymeric nanoplatform has been developed for the targeted delivery of EZH2-targeted CRISPR/Cas9 system in prostate cancer treatment, achieving efficient EZH2 gene disruption and significant downregulation of EZH2 protein expression. The system also led to enhanced apoptosis through H3K27me3 downregulation, resulting in substantial inhibition of prostate cancer cell proliferation and growth repression of subcutaneous xenograft tumors. This innovative nanoplatform not only provides a prospective nanocarrier for CRISPR/Cas9 system delivery, but also broadens the potential of combining gene editing-chemo synergistic cancer therapy.

NANO RESEARCH (2021)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Liposomal delivery of CRISPR/Cas9

Shuai Zhen et al.

CANCER GENE THERAPY (2020)

添加到收藏夹

Review Nanoscience & Nanotechnology

Strategies for nonviral nanoparticle-based delivery of CRISPR/Cas9 therapeutics

Fengqian Chen et al.

WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

Peter Istvan Kulcsar et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Engineering, Biomedical

High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

Hui Kwon Kim et al.

NATURE BIOMEDICAL ENGINEERING (2020)

添加到收藏夹

Article Multidisciplinary Sciences

The effects of electroporation buffer composition on cell viability and electro-transfection efficiency

Joseph J. Sherba et al.

SCIENTIFIC REPORTS (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Hyperbranched poly(β-amino ester) based polyplex nanopaticles for delivery of CRISPR/Cas9 system and treatment of HPV infection associated cervical cancer

Xueqin Gao et al.

JOURNAL OF CONTROLLED RELEASE (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Polyethylenimine based magnetic nanoparticles mediated non-viral CRISPR/Cas9 system for genome editing

S. S. Rohiwal et al.

SCIENTIFIC REPORTS (2020)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR screening identifies M1AP as a new MYC regulator with a promoter-reporter system

Akiko Yamamoto et al.

PEERJ (2020)

添加到收藏夹

Article Biotechnology & Applied Microbiology

AAV-CRISPR Gene Editing Is Negated by Pre-existing Immunity to Cas9

Ang Li et al.

MOLECULAR THERAPY (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Engineered Interactions with Mesoporous Silica Facilitate Intracellular Delivery of Proteins and Gene Editing

Bin Liu et al.

NANO LETTERS (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer

Qingfei Zhang et al.

NANO LETTERS (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer

You Lu et al.

NATURE MEDICINE (2020)

添加到收藏夹

Review Biochemistry & Molecular Biology

Recent Advances in CRISPR/Cas9 Delivery Strategies

Bon Ham Yip

BIOMOLECULES (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

AAV-mediated FOXG1 gene editing in human Rett primary cells

Susanna Croci et al.

EUROPEAN JOURNAL OF HUMAN GENETICS (2020)

添加到收藏夹

Article Pharmacology & Pharmacy

Polyplexes for gene and nucleic acid delivery: Progress and bottlenecks

Kevin Ita

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2020)

添加到收藏夹

Article Medicine, Research & Experimental

AAV-Mediated CRISPR/Cas9 Gene Editing in Murine Phenylketonuria

Daelyn Y. Richards et al.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2020)

添加到收藏夹

Article Ophthalmology

Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption

Hossein Ameri et al.

TRANSLATIONAL VISION SCIENCE & TECHNOLOGY (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Codelivery of CRISPR-Cas9 and chlorin e6 for spatially controlled tumor-specific gene editing with synergistic drug effects

Shaohui Deng et al.

SCIENCE ADVANCES (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

A pH-responsive silica-metal-organic framework hybrid nanoparticle for the delivery of hydrophilic drugs, nucleic acids, and CRISPR-Cas9 genome-editing machineries

Yuyuan Wang et al.

JOURNAL OF CONTROLLED RELEASE (2020)

添加到收藏夹

Article Engineering, Biomedical

MicroRNA-responsive release of Cas9/sgRNA from DNA nanoflower for cytosolic protein delivery and enhanced genome editing

Jinjin Shi et al.

BIOMATERIALS (2020)

添加到收藏夹

Review Biochemistry & Molecular Biology

Application of Various Delivery Methods for CRISPR/dCas9

Zhixi Liu et al.

MOLECULAR BIOTECHNOLOGY (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo

Khalid A. Hajj et al.

NANO LETTERS (2020)

添加到收藏夹

Article Chemistry, Physical

Light-responsive charge-reversal nanovector for high-efficiencyin vivoCRISPR/Cas9 gene editing with controllable location and time

Yunxia Wu et al.

NANO RESEARCH (2020)

添加到收藏夹

Article Biochemical Research Methods

Design, fabrication and applications of tetrahedral DNA nanostructure-based multifunctional complexes in drug delivery and biomedical treatment

Tao Zhang et al.

NATURE PROTOCOLS (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

Tuo Wei et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Review Biochemistry & Molecular Biology

Methods Favoring Homology-Directed Repair Choice in Response to CRISPR/Cas9 Induced-Double Strand Breaks

Han Yang et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

添加到收藏夹

Review Biochemistry & Molecular Biology

CRISPR-Cas9 DNA Base-Editing and Prime-Editing

Ariel Kantor et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

添加到收藏夹

Review Nanoscience & Nanotechnology

Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications

Yi Zhang et al.

INTERNATIONAL JOURNAL OF NANOMEDICINE (2020)

添加到收藏夹

Article Biochemical Research Methods

GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos

Erwei Zuo et al.

NATURE PROTOCOLS (2020)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR/Cas9-mediated correction of mutated copper transporter ATP7B

Michael Poehler et al.

PLOS ONE (2020)

添加到收藏夹

Article Oncology

Knockout of EGFL6 by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer

Wenhui Zhu et al.

FRONTIERS IN ONCOLOGY (2020)

添加到收藏夹

Article Nanoscience & Nanotechnology

Co-delivery of Sorafenib and CRISPR/Cas9 Based on Targeted Core-Shell Hollow Mesoporous Organosilica Nanoparticles for Synergistic HCC Therapy

Bing-Chen Zhang et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

添加到收藏夹

Review Medicine, Research & Experimental

CRISPR/Cas9: A powerful genome editing technique for the treatment of cancer cells with present challenges and future directions

Abu Hazafa et al.

LIFE SCIENCES (2020)

添加到收藏夹

Article Multidisciplinary Sciences

A simple method using CRISPR-Cas9 to knock-out genes in murine cancerous cell lines

Airi Ishibashi et al.

SCIENTIFIC REPORTS (2020)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Expanding application of CRISPR-Cas9 system in microorganisms

Jing Zhao et al.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2020)

添加到收藏夹

Review Chemistry, Multidisciplinary

Key considerations in designing CRISPR/Cas9-carrying nanoparticles for therapeutic genome editing

Yunxue Xu et al.

NANOSCALE (2020)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy

Daniel Rosenblum et al.

SCIENCE ADVANCES (2020)

添加到收藏夹

Article Agronomy

Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

Feipeng Wang et al.

CROP JOURNAL (2020)

添加到收藏夹

Article Materials Science, Biomaterials

An engineered exosome for delivering sgRNA:Cas9 ribonucleoprotein complex and genome editing in recipient cells

Yangyang Ye et al.

BIOMATERIALS SCIENCE (2020)

添加到收藏夹

Review Medicine, Research & Experimental

Micro/nano-bubble-assisted ultrasound to enhance the EPR effect and potential theranostic applications

Lei Duan et al.

THERANOSTICS (2020)

添加到收藏夹

Article Plant Sciences

SpCas9-NG self-targets the sgRNA sequence in plant genome editing

Ruiying Qin et al.

NATURE PLANTS (2020)

添加到收藏夹

Article Robotics

Automated High-Productivity Microinjection System for Adherent Cells

Fei Pan et al.

IEEE ROBOTICS AND AUTOMATION LETTERS (2020)

添加到收藏夹

Review Oncology

CRISPR/Cas9 for cancer research and therapy

Tianzuo Zhan et al.

SEMINARS IN CANCER BIOLOGY (2019)

添加到收藏夹

Review Biochemical Research Methods

Harnessing Exosomes for the Development of Brain Drug Delivery Systems

Mengna Zheng et al.

BIOCONJUGATE CHEMISTRY (2019)

添加到收藏夹

Article Medicine, Research & Experimental

CRISPR/Cas9-mediated knockout of the PDEF gene inhibits migration and invasion of human gastric cancer AGS cells

Yi-Qiang Zhang et al.

BIOMEDICINE & PHARMACOTHERAPY (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9

Calvin J. Stephens et al.

JOURNAL OF CONTROLLED RELEASE (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Nanoscale ATP-Responsive Zeolitic Imidazole Framework-90 as a General Platform for Cytosolic Protein Delivery and Genome Editing

Xiaoti Yang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Engineering of Bioinspired, Size-Controllable, Self-Degradable Cancer-Targeting DNA Nanoflowers via the Incorporation of an Artificial Sandwich Base

Lili Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Clinically Relevant Correction of Recessive Dystrophic Epidermolysis Bullosa by Dual sgRNA CRISPR/Cas9-Mediated Gene Editing

Jose Bonafont et al.

MOLECULAR THERAPY (2019)

添加到收藏夹

Article Biochemistry & Molecular Biology

Identification of preexisting adaptive immunity to Cas9 proteins in humans

Carsten T. Charlesworth et al.

NATURE MEDICINE (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Lecithin nano-liposomal particle as a CRISPR/Cas9 complex delivery system for treating type 2 diabetes

Eun Yi Cho et al.

JOURNAL OF NANOBIOTECHNOLOGY (2019)

添加到收藏夹

Article Medicine, Research & Experimental

A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing

Ang Li et al.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2019)

添加到收藏夹

Article Cell Biology

Optimizing genome editing strategy by primer-extension-mediated sequencing

Jianhang Yin et al.

CELL DISCOVERY (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

A Rationally Designed Semiconducting Polymer Brush for NIR-II Imaging-Guided Light-Triggered Remote Control of CRISPR/Cas9 Genome Editing

Ling Li et al.

ADVANCED MATERIALS (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

In Vitro Validation of a CRISPR-Mediated CFTR Correction Strategy for Preclinical Translation in Pigs

Zhichang Peter Zhou et al.

HUMAN GENE THERAPY (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Lentiviral CRISPR Epigenome Editing of Inflammatory Receptors as a Gene Therapy Strategy for Disc Degeneration

Niloofar Farhang et al.

HUMAN GENE THERAPY (2019)

添加到收藏夹

Article Biochemical Research Methods

Genome-wide CRISPR-Cas9 screening in mammalian cells

Jason S. L. Yu et al.

METHODS (2019)

添加到收藏夹

Review Cell Biology

A CRISPR/Cas9 based polymeric nanoparticles to treat/inhibit microbial infections

Richa Verma et al.

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Impact of CXCR4 and CXCR7 knockout by CRISPR/Cas9 on the function of triple-negative breast cancer cells

Meng Yang et al.

ONCOTARGETS AND THERAPY (2019)

添加到收藏夹

Article Oncology

A functional CRISPR/Cas9 screen identifies kinases that modulate FGFR inhibitor response in gastric cancer

Jiamin Chen et al.

ONCOGENESIS (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles

Ji Liu et al.

ADVANCED MATERIALS (2019)

添加到收藏夹

Article Biochemistry & Molecular Biology

Construction of Traf3 knockout liver cancer cell line using CRISPR/Cas9 system

Wenbo Hu et al.

JOURNAL OF CELLULAR BIOCHEMISTRY (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Knockdown of hypoxia-inducible factor-1 alpha by tumor targeted delivery of CRISPR/Cas9 system suppressed the metastasis of pancreatic cancer

Man Li et al.

JOURNAL OF CONTROLLED RELEASE (2019)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Delivery of CRISPR/Cas9 for therapeutic genome editing

Xiaojie Xu et al.

JOURNAL OF GENE MEDICINE (2019)

添加到收藏夹

Article Medicine, Research & Experimental

Production of CRISPR/Cas9-Mediated Self-Cleaving Helper-Dependent Adenoviruses

Donna J. Palmer et al.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2019)

添加到收藏夹

Article Multidisciplinary Sciences

A boronic acid-rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

Chongyi Liu et al.

SCIENCE ADVANCES (2019)

添加到收藏夹

Article Engineering, Biomedical

Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy

Ana M. Moreno et al.

NATURE BIOMEDICAL ENGINEERING (2019)

添加到收藏夹

Article Nanoscience & Nanotechnology

Gold Nanocluster-Mediated Efficient Delivery of Cas9 Protein through pH-Induced Assembly-Disassembly for Inactivation of Virus Oncogenes

Enguo Ju et al.

ACS APPLIED MATERIALS & INTERFACES (2019)

添加到收藏夹

Article Biochemistry & Molecular Biology

Robotic platform for microinjection into single cells in brain tissue

Gabriella Shull et al.

EMBO REPORTS (2019)

添加到收藏夹

Review Biochemistry & Molecular Biology

Applications of CRISPR/Cas9 Technology in the Treatment of Lung Cancer

Chunyang Jiang et al.

TRENDS IN MOLECULAR MEDICINE (2019)

添加到收藏夹

Article Nanoscience & Nanotechnology

Cell-Selective Messenger RNA Delivery and CRISPR/Cas9 Genome Editing by Modulating the Interface of Phenylboronic Acid-Derived Lipid Nanoparticles and Cellular Surface Sialic Acid

Qiao Tang et al.

ACS APPLIED MATERIALS & INTERFACES (2019)

添加到收藏夹

Article Multidisciplinary Sciences

Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Josh Tycko et al.

NATURE COMMUNICATIONS (2019)

添加到收藏夹

Review Nanoscience & Nanotechnology

Engineered materials for in vivo delivery of genome-editing machinery

Sheng Tong et al.

NATURE REVIEWS MATERIALS (2019)

添加到收藏夹

Article Multidisciplinary Sciences

Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing

Yuan Rui et al.

SCIENCE ADVANCES (2019)

添加到收藏夹

Article Chemistry, Multidisciplinary

Thermo-triggered Release of CRISPR-Cas9 System by Lipid-Encapsulated Gold Nanoparticles for Tumor Therapy

Peng Wang et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

添加到收藏夹

Review Engineering, Biomedical

Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities

Ling Li et al.

BIOMATERIALS (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance

Assaf C. Bester et al.

CELL (2018)

添加到收藏夹

Review Pharmacology & Pharmacy

Use of CRISPR/Cas9 gene-editing tools for developing models in drug discovery

Gulzar Ahmad et al.

DRUG DISCOVERY TODAY (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

No off-target mutations in functional genome regions of a CRISPR/Cas9-generated monkey model of muscular dystrophy

Shuang Wang et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2018)

添加到收藏夹

Article Nanoscience & Nanotechnology

Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers

Alexander S. Timin et al.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE (2018)

添加到收藏夹

Article Biotechnology & Applied Microbiology

A highly specific SpCas9 variant is identified by in vivo screening in yeast

Antonio Casini et al.

NATURE BIOTECHNOLOGY (2018)

添加到收藏夹

Article Multidisciplinary Sciences

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

Simon E. Troeder et al.

PLOS ONE (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide

Hong-Xia Wang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

添加到收藏夹

Review Chemistry, Analytical

Recent biomedical applications of gold nanoparticles: A review

Narges Elahi et al.

TALANTA (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Efficient RNA drug delivery using red blood cell extracellular vesicles

Waqas Muhammad Usman et al.

NATURE COMMUNICATIONS (2018)

添加到收藏夹

Article Cell Biology

Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication

Julianna Han et al.

CELL REPORTS (2018)

添加到收藏夹

Article Geriatrics & Gerontology

Genome-editing applications of CRISPR-Cas9 to promote in vitro studies of Alzheimer's disease

Vo Van Giau et al.

CLINICAL INTERVENTIONS IN AGING (2018)

添加到收藏夹

Article Medicine, Research & Experimental

One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors

Maren Schiwon et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2018)

添加到收藏夹

Article Oncology

Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

Freddie Bray et al.

CA-A CANCER JOURNAL FOR CLINICIANS (2018)

添加到收藏夹

Article Multidisciplinary Sciences

In vivo CRISPR editing with no detectable genome-wide off-target mutations

Pinar Akcakaya et al.

NATURE (2018)

添加到收藏夹

Review Multidisciplinary Sciences

CRISPR-Cas guides the future of genetic engineering

Gavin J. Knott et al.

SCIENCE (2018)

添加到收藏夹

Article Cell & Tissue Engineering

Genetically Engineered Adipose Mesenchymal Stem Cells Using HIV-Based Lentiviral Vectors as Gene Therapy for Autoimmune Diseases

Masoumeh Rostami et al.

CELLULAR REPROGRAMMING (2018)

添加到收藏夹

Review Obstetrics & Gynecology

CRISPR/Cas9 gene-editing: Research technologies, clinical applications and ethical considerations

Fani Memi et al.

SEMINARS IN PERINATOLOGY (2018)

添加到收藏夹

Article Medicine, Research & Experimental

Overcoming the Undesirable CRISPR-Cas9 Expression in Gene Correction

Emily Xia et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2018)

添加到收藏夹

Article Multidisciplinary Sciences

New CRISPR-Cas systems from uncultivated microbes

David Burstein et al.

NATURE (2017)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Cornerstones of CRISPR-Cas in drug discovery and therapy

Christof Fellmann et al.

NATURE REVIEWS DRUG DISCOVERY (2017)

添加到收藏夹

Article Gastroenterology & Hepatology

Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice

Chun-Qing Song et al.

GASTROENTEROLOGY (2017)

添加到收藏夹

Article Chemistry, Multidisciplinary

Cancer-derived exosomes as a delivery platform of CRISPR/Cas9 confer cancer cell tropism-dependent targeting

Seung Min Kim et al.

JOURNAL OF CONTROLLED RELEASE (2017)

添加到收藏夹

Article Biochemistry & Molecular Biology

A Novel Rat Model of Nonalcoholic Fatty Liver Disease Constructed Through CRISPR/Cas-Based Hydrodynamic Injection

Qin Yu et al.

MOLECULAR BIOTECHNOLOGY (2017)

添加到收藏夹

Article Multidisciplinary Sciences

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

Sushrut Kamerkar et al.

NATURE (2017)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

Hao Yin et al.

NATURE BIOTECHNOLOGY (2017)

添加到收藏夹

Review Biochemical Research Methods

Genome editing in livestock: Are we ready for a revolution in animal breeding industry?

Jinxue Ruan et al.

TRANSGENIC RESEARCH (2017)

添加到收藏夹

Article Chemistry, Multidisciplinary

Synthetically controlling dendrimer flexibility improves delivery of large plasmid DNA

Jessica A. Kretzmann et al.

CHEMICAL SCIENCE (2017)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep

Marcela Vilarino et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Article Multidisciplinary Sciences

A CRISPR/Cas9 guidance RNA screen platform for HIV provirus disruption and HIV/AIDS gene therapy in astrocytes

Zaohua Huang et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes

Eric Ehrke-Schulz et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Review Chemistry, Multidisciplinary

CRISPR/Cas9-Based Genome Editing for Disease Modeling and Therapy: Challenges and Opportunities for Nonviral Delivery

Hong-Xia Wang et al.

CHEMICAL REVIEWS (2017)

添加到收藏夹

Review Biochemistry & Molecular Biology

Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine

Cody S. Lee et al.

GENES & DISEASES (2017)

添加到收藏夹

Review Cell Biology

Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation

Antonia A. Dominguez et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2016)

添加到收藏夹

Article Pharmacology & Pharmacy

Hydrodynamic Tail Vein Injection as a Simple Tool for Yielding Extended Transgene Expression in Solid Tumors

Takuma Takayama et al.

BIOLOGICAL & PHARMACEUTICAL BULLETIN (2016)

添加到收藏夹

Review Biochemistry & Molecular Biology

The Heroes of CRISPR

Eric S. Lander

CELL (2016)

添加到收藏夹

Review Chemistry, Multidisciplinary

Gated Materials for On-Command Release of Guest Molecules

Elena Aznar et al.

CHEMICAL REVIEWS (2016)

添加到收藏夹

Article Automation & Control Systems

A High-Throughput Automated Microinjection System for Human Cells With Small Size

Yu Ting Chow et al.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2016)

添加到收藏夹

Review Chemistry, Multidisciplinary

Gene therapy for the CNS using AAVs: The impact of systemic delivery by AAV9

Joana Saraiva et al.

JOURNAL OF CONTROLLED RELEASE (2016)

添加到收藏夹

Article Biochemical Research Methods

A multifunctional AAV-CRISPR-Cas9 and its host response

Wei Leong Chew et al.

NATURE METHODS (2016)

添加到收藏夹

Article Multidisciplinary Sciences

Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM

Alan S. L. Wong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

添加到收藏夹

Review Chemistry, Applied

Engineering Delivery Vehicles for Genome Editing

Christopher E. Nelson et al.

ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 7 (2016)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis

Thomas Naert et al.

SCIENTIFIC REPORTS (2016)

添加到收藏夹

Article Oncology

The kinome 'at large' in cancer

Emmy D. G. Fleuren et al.

NATURE REVIEWS CANCER (2016)

添加到收藏夹

Article Chemistry, Multidisciplinary

Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR-Cas9 for Genome Editing

Wujin Sun et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

添加到收藏夹

Article Biochemistry & Molecular Biology

Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

Sidi Chen et al.

CELL (2015)

添加到收藏夹

Review Biotechnology & Applied Microbiology

The therapeutic application of CRISPR/Cas9 technologies for HIV

Sheena Saayman et al.

EXPERT OPINION ON BIOLOGICAL THERAPY (2015)

添加到收藏夹

Article Genetics & Heredity

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

Wenning Qin et al.

GENETICS (2015)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

Xiquan Liang et al.

JOURNAL OF BIOTECHNOLOGY (2015)

添加到收藏夹

Review Neurosciences

CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases

Zhuchi Tu et al.

MOLECULAR NEURODEGENERATION (2015)

添加到收藏夹

Editorial Material Biotechnology & Applied Microbiology

Nanomedical innovation: the SEON-concept for an improved cancer therapy with magnetic nanoparticles

Stefan Lyer et al.

NANOMEDICINE (2015)

添加到收藏夹

Review Microbiology

An updated evolutionary classification of CRISPR-Cas systems

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2015)

添加到收藏夹

Review Medicine, General & Internal

Inflammatory Muscle Diseases

Marinos C. Dalakas

NEW ENGLAND JOURNAL OF MEDICINE (2015)

添加到收藏夹

Article Genetics & Heredity

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

Wenning Qin et al.

GENETICS (2015)

添加到收藏夹

Review Medicine, Research & Experimental

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

Xiao-Hui Zhang et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2015)

添加到收藏夹

Article Multidisciplinary Sciences

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Masakazu Hashimoto et al.

SCIENTIFIC REPORTS (2015)

添加到收藏夹

Article Cell Biology

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

Xingjie Ren et al.

CELL REPORTS (2014)

添加到收藏夹

Article Medicine, Research & Experimental

Hemodynamics of a hydrodynamic injection

Tsutomu Kanefuji et al.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2014)

添加到收藏夹

Article Biochemistry & Molecular Biology

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Luke A. Gilbert et al.

CELL (2013)

添加到收藏夹

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

添加到收藏夹

Review Oncology

Kinesins and cancer

Oliver Rath et al.

NATURE REVIEWS CANCER (2012)

添加到收藏夹

Editorial Material Multidisciplinary Sciences

Unique structure and property of dendrimers in biomedical applications INTRODUCTION

Minghui Chai

PROCEEDINGS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES (2010)

添加到收藏夹

Article Immunology

Examining attitudes and knowledge about HPV and cervical cancer risk among female clinic attendees in Johannesburg, South Africa

Shelley A. Francis et al.

VACCINE (2010)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

添加到收藏夹

Review Immunology

Immune privilege induced by regulatory T cells in transplantation tolerance

Stephen P. Cobbold et al.

IMMUNOLOGICAL REVIEWS (2006)

添加到收藏夹

Article Biochemistry & Molecular Biology

Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-α

MRM Fontes et al.

JOURNAL OF MOLECULAR BIOLOGY (2000)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.