期刊
CELLS
卷 12, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/cells12172131
关键词
glaucoma; astrocytes; optic nerve head; RNA-sequencing; phagocytosis; mitophagy
类别
The optic nerve head is believed to be the initial site of retinal ganglion cell injury in glaucoma. Astrocytes in the optic nerve head respond to injury by undergoing characteristic changes in cell morphology and gene expression profile. Increased intraocular pressure leads to upregulation of astrocytic phagocytosis, indicating its involvement in the early stages of glaucoma.
The optic nerve head is thought to be the site of initial injury to retinal ganglion cell injury in glaucoma. In the initial segment of the optic nerve directly behind the globe, the ganglion cell axons are unmyelinated and come into direct contact to astrocytes, suggesting that astrocytes may play a role in the pathology of glaucoma. As in other parts of the CNS, optic nerve head astrocytes respond to injury by characteristic changes in cell morphology and gene expression profile. Using RNA-sequencing of glaucomatous optic nerve heads, single-cell PCR, and an in-vivo assay, we demonstrate that an up-regulation of astrocytic phagocytosis is an early event after the onset of increased intraocular pressure. We also show that astrocytes in the glial lamina of the optic nerve are apparently functionally heterogeneous. At any time, even in naive nerves, some of the cells show signs of reactivity-process hypertrophy, high phagocytic activity, and expression of genetic markers of reactivity whereas neighboring cells apparently are inactive. A period of increased intraocular pressure moves more astrocytes towards the reactive phenotype; however, some cells remain unreactive even in glaucomatous nerves.
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