Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

Dental pulp stem cells (DPSCs) are considered a valuable cell source for regenerative medicine because of their high proliferative potential, multipotency, and availability. We established a new cryopreservation method (NCM) for collecting DPSCs, in which the tissue itself is cryopreserved and DPSCs are collected after thawing. We improved the NCM and developed a new method for collecting and preserving DPSCs more efficiently. Dental pulp tissue was collected from an extracted tooth, divided into two pieces, sandwiched from above and below using cell culture inserts, and cultured. As a result, the cells in the pulp tissue migrated vertically over time and localized near the upper and lower membranes over 2-3 days. With regard to the underlying molecular mechanism, SDF1 was predominantly involved in cell migration. This improved method is valuable and enables the more efficient collection and reliable preservation of DPSCs. It has the potential to procure a large number of DPSCs stably.

Automated summary

Dental pulp stem cells (DPSCs) are highly valuable for regenerative medicine due to their proliferative potential and multipotency. A new cryopreservation method (NCM) was developed to collect DPSCs by freezing the tissue and collecting the cells after thawing. An improved method was developed to efficiently collect and preserve DPSCs by culturing dental pulp tissue with cell culture inserts, resulting in vertical cell migration and localization near the membrane. The underlying molecular mechanism involves the predominance of SDF1 in cell migration.