期刊
CELLS
卷 12, 期 16, 页码 -出版社
MDPI
DOI: 10.3390/cells12162087
关键词
rat; dorsal hippocampus; neurodegeneration; Alzheimer's disease-like pathology; methionine diet; H-1 MRS
类别
This study investigates the effects of a high methionine diet on metabolic ratio and histomorphological alterations in the rat hippocampus. The results show that high methionine diet-induced hyperhomocysteinemia leads to significant changes in metabolism and tissue morphology in the hippocampus. These changes may contribute to the progression of neurodegeneration and the development of Alzheimer's disease-like pathological features.
Multifactorial interactions, including nutritional state, likely participate in neurodegeneration's pathogenesis and evolution. Dysregulation in methionine (Met) metabolism could lead to the development of hyperhomocysteinaemia (hHcy), playing an important role in neuronal dysfunction, which could potentially lead to the development of Alzheimer's disease (AD)-like pathological features. This study combines proton magnetic resonance spectroscopy (1H MRS) with immunohistochemical analysis to examine changes in the metabolic ratio and histomorphological alterations in the dorsal rat hippocampus (dentate gyrus-DG) subjected to a high Met diet. Male Wistar rats (420-480 g) underwent hHcy evoked by a Met-enriched diet (2 g/kg of weight/day) lasting four weeks. Changes in the metabolic ratio profile and significant histomorphological alterations have been found in the DG of hHcy rats. We have detected increased morphologically changed neurons and glial cells with increased neurogenic markers and apolipoprotein E positivity parallel with a diminished immunosignal for the N-Methyl-D-Aspartate receptor 1 in hHcy animals. A Met diet induced hHcy, likely via direct Hcy neurotoxicity, an interference with one carbon unit metabolism, and/or epigenetic regulation. These conditions lead to the progression of neurodegeneration and the promotion of AD-like pathological features in the less vulnerable hippocampal DG, which presents a plausible therapeutic target.
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