4.6 Article

Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis

期刊

CELLS
卷 12, 期 17, 页码 -

出版社

MDPI
DOI: 10.3390/cells12172195

关键词

systemic sclerosis; lymphatic endothelial cells; endothelial-to-myofibroblast transition; myofibroblasts; skin; fibrosis

向作者/读者索取更多资源

Currently, only a few studies have investigated the role of the lymphatic vascular system in the pathogenesis of systemic sclerosis (SSc). This study aimed to explore the possibility of lymphatic endothelial cells undergoing an endothelial-to-myofibroblast transition (Ly-EndMT) and contributing to skin fibrosis in SSc. The results demonstrated the presence of lymphatic endothelial cells in intermediate stages of the Ly-EndMT process exclusively in the fibrotic skin of SSc patients, suggesting that Ly-EndMT might be a previously overlooked pathogenic process linking peripheral microlymphatic dysfunction and skin fibrosis development in SSc.
At present, only a few reports have addressed the possible contribution of the lymphatic vascular system to the pathogenesis of systemic sclerosis (SSc). Based on the evidence that blood vascular endothelial cells can undertake the endothelial-to-myofibroblast transition (EndMT) contributing to SSc-related skin fibrosis, we herein investigated whether the lymphatic endothelium might represent an additional source of profibrotic myofibroblasts through a lymphatic EndMT (Ly-EndMT) process. Skin sections from patients with SSc and healthy donors were immunostained for the lymphatic endothelial cell-specific marker lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) in combination with & alpha;-smooth muscle actin (& alpha;-SMA) as the main marker of myofibroblasts. Commercial human adult dermal lymphatic microvascular endothelial cells (HdLy-MVECs) were challenged with recombinant human transforming growth factor-& beta;1 (TGF & beta;1) or serum from SSc patients and healthy donors. The expression of lymphatic endothelial cell/myofibroblast markers was measured by quantitative real-time PCR, Western blotting and immunofluorescence. Collagen gel contraction assay was performed to assess myofibroblast-like cell contractile ability. Lymphatic endothelial cells in intermediate stages of the Ly-EndMT process (i.e., coexpressing LYVE-1 and & alpha;-SMA) were found exclusively in the fibrotic skin of SSc patients. The culturing of HdLy-MVECs with SSc serum or profibrotic TGF & beta;1 led to the acquisition of a myofibroblast-like morphofunctional phenotype, as well as the downregulation of lymphatic endothelial cell-specific markers and the parallel upregulation of myofibroblast markers. In SSc, the Ly-EndMT might represent a previously overlooked pathogenetic process bridging peripheral microlymphatic dysfunction and skin fibrosis development.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据