期刊
ACS SENSORS
卷 8, 期 11, 页码 3988-4007出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.3c01338
关键词
Point-of-care; CRISPR/Cas12a; CRISPRsensing; Biosensor; Signal output; Portabledetectiondevices; Rapid detection; Diagnosis
This review article focuses on the signal readout and sensitivity-improving strategies of CRISPR/Cas12a biosensors, and introduces innovative applications of portability strategies.
Point-of-care (POC) detection is getting more and more attention in many fields due to its accuracy and on-site test property. The CRISPR/Cas12a system is endowed with excellent sensitivity, target identification specificity, and signal amplification ability in biosensing because of its unique trans-cleavage ability. As a result, a lot of research has been made to develop CRISPR/Cas12a-based biosensors. In this review, we focused on signal readout strategies and summarized recent sensitivity-improving strategies in fluorescence, colorimetric, and electrochemical signaling. Then we introduced novel portability-improving strategies based on lateral flow assays (LFAs), microfluidic chips, simplified instruments, and one-pot design. In the end, we also provide our outlook for the future development of CRISPR/Cas12a biosensors.
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