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Article
Biochemical Research Methods
Claudio Parolo et al.
Summary: The functionality of lab on a chip (LOC) devices has expanded in recent years, providing scientists and developers with versatile platforms for various chemical and biological applications. However, real-time molecular measurements, which are crucial for guiding chemical responses, are still lacking in this field. Among the general sensing strategies, those relying on binding-induced conformational change of bioreceptors are expected to be the most successful in providing LOCs with real-time molecular monitoring capabilities.
Review
Chemistry, Analytical
Esther Sanchez-Tirado et al.
Summary: The study of the human microbiome is a multidisciplinary field that holds great potential for improving disease diagnosis and monitoring using microbiota biomarkers. Electrochemical biosensors, with their high sensitivity and fast response, combined with advancements in nanomaterials, offer unique tools for understanding the human microbiome and driving future developments. This review article focuses on the application of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers in the gastrointestinal microbiome, discussing challenges and future directions.
Article
Biochemistry & Molecular Biology
Maria Gamella et al.
Summary: A disposable electrochemical PCR-free biosensor was developed for the selective detection of Sin a 1, a diagnostic marker for mustard sensitization. The biosensor utilized DNA/RNA heterohybrids formed by sandwich hybridization with specific RNA probes, and employed antibodies and horseradish peroxidase for labeling. The biosensor achieved fast and simple detection of Sin a 1 with a limit of detection of 3 pM, using just 50 ng of non-fragmented denatured genomic DNA from yellow mustard seeds.
BIOELECTROCHEMISTRY
(2023)
Review
Biochemical Research Methods
Diana-Gabriela Macovei et al.
Summary: Recent years have shown the benefits of diagnosing and monitoring biomarkers related to inflammatory medical conditions in improving healthcare and patient life quality. The development of integrated and portable point-of-care testing devices for early disease diagnosis and monitoring, even at the patient's bed, is crucial. This review focuses on the progress in detecting specific biomarkers and inflammation-related biomarkers in various diseases and provides an overview of the latest electrochemical sensors for their detection. The future perspectives of point-of-care testing to enhance healthcare management are also discussed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Shaoguang Li et al.
Summary: Whole blood, as an important biological fluid, provides crucial information for health management and disease monitoring. In the past decade, advancements in nanotechnology, microfluidics, and biomarker research have led to the development of miniaturized diagnostic systems for whole blood testing. Electrochemical biosensors have emerged as a promising technology for detecting specific targets in complex media, such as whole blood or the human body, due to their rapidity, sensitivity, miniaturization capability, reagentless nature, and washing-free features. This review aims to summarize the progress made in the development of electrochemical sensors for whole blood analysis in the last decade, while addressing the challenges and opportunities in integrating electrochemical sensing platforms.
Review
Chemistry, Analytical
Susana Campuzano et al.
Summary: The role of liquid biopsy in precision, equitable, and sustainable diagnosis of prevalent and highly heterogeneous diseases using biomarkers derived from whole cells, secretomes, and exosomes is undeniable. Electrochemical biosensing and biosensors, particularly those involving affinity reactions, have emerged as competitive and cost-effective analytical alternatives for identifying these challenging targets. This review provides a concise and up-to-date overview of the fundamentals, versatility, and opportunities offered by state-of-the-art electrochemical biosensing (2018-2022) for the determination of cellular-derived biomarkers. It also offers critical insights into the short-term challenges and future research directions in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Huyan Xiao et al.
Summary: The evolving SARS-CoV-2 virus has produced various variants, including concern variants (VOCs). CRISPR-Cas systems have been utilized to develop techniques for detecting these variants, focusing on variant-specific mutations in the spike protein gene of SARS-CoV-2. Differentiating these sequences, which mostly consist of single-nucleotide mutations, is challenging with a single CRISPR-based assay. This article discusses the specificity of Cas9, Cas12, and Cas13 systems, important considerations for mutation sites, guide RNA design, and recent advancements in CRISPR-based assays for SARS-CoV-2 variants. Strategies for distinguishing single-nucleotide mutations include optimizing mismatch positions, modifying nucleotides in the guide RNA, and utilizing two guide RNAs for recognizing the specific mutation sequence and a conservative sequence. Further research is necessary to address challenges in detecting and differentiating SARS-CoV-2 variants and sublineages in clinical diagnostic and point-of-care applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Genetics & Heredity
Mahya Ahmadpour Youshanlui et al.
Article
Oncology
Ana Montero-Calle et al.
Summary: The study aimed to assess whether p53 and p63 proteoforms derived from alternative splicing could have a differential seroreactivity and higher diagnostic value than canonical proteins in colorectal cancer. The researchers demonstrated the appearance of a differential seroreactivity among the proteoforms in colorectal cancer patients. These findings reveal increased complexity of the humoral immune response in cancer and highlight the potential for developing a blood-based biomarker signature for colorectal cancer detection.
Article
Biochemical Research Methods
Gabriela F. Giordano et al.
Summary: The fourth paradigm in science, using machine learning, has made its way into the sensing area. It has shown significant impact in improving sensitivity, reproducibility, and accuracy, as well as enabling the determination of multiple targets from a single measurement. By training supervised machine learning models on large datasets produced by electrical and electrochemical bio/sensors, it has become possible to overcome typical issues such as electrode fouling, poor signal-to-noise ratio, chemical interferences, and matrix effects. This trend article not only provides an outlook for the future but also presents examples from the literature to demonstrate how machine learning algorithms can help address these interfering issues and enable the translation of testing technologies into practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Jiaobing Tu et al.
Summary: A wearable electrochemical patch has been developed to monitor the biomarker C-reactive protein in sweat in real-time, allowing for point-of-care monitoring in patients with acute or chronic inflammation. The patch integrates various components such as iontophoretic sweat extraction, microfluidic channels for sampling and reagent routing, and a graphene-based sensor array for quantifying CRP and calibrating the sensor with ionic strength, pH, and temperature. The patch showed good correlation with serum protein levels in patients with chronic diseases.
NATURE BIOMEDICAL ENGINEERING
(2023)
Review
Chemistry, Physical
Madalin Alexandru Cobzariu et al.
Summary: The detection of genetic material is crucial in clinical practice, food quality control, and environmental analysis. Nucleic acid tests involve amplification to detect the low amount of target sequences, with enzyme-assisted isothermal amplification strategies such as HDA, LAMP, and RPA being easier to adapt to electrochemical platforms for point-of-need applications compared to PCR. This review discusses the current state and challenges of coupling these amplification strategies to conductive platforms for electrochemical transduction in real-world point-of-need tests.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Tongtong Zhang et al.
Summary: This study developed a low-cost and stable electrochemical aptasensor using vertically-ordered mesoporous silica films (VMSF) for highly sensitive and direct detection of alpha fetoprotein (AFP) in human serum, which is vital for early diagnosis of hepatocellular carcinoma. VMSF, with silanol groups on the surface and regularly ordered nanopores, provides binding sites for functionalization of recognition aptamer and also possesses excellent anti-biofouling capacity. The sensing mechanism relies on the target AFP-controlled diffusion of Fe(CN)(6)(3-/4-) redox electrochemical probe through the nanochannels of VMSF, resulting in reduced electrochemical responses that are related to the AFP concentration. The developed aptasensor demonstrates a wide dynamic linear range and a low limit of detection of AFP.
Editorial Material
Chemistry, Analytical
Ieva Plikusiene et al.
Article
Biochemistry & Molecular Biology
Wanwisa Deenin et al.
Summary: In this study, a new highly sensitive and selective COVID-19 diagnostic device was developed by combining the principle of ATKs with electrochemical detection, allowing quantification using a smartphone. The device utilized a screen-printed electrode inside a lateral flow device to exploit the binding affinity between SARS-CoV-2 antigen and ACE2. Electrochemical assay signal intensity on smartphones increased proportionally to the concentration of SARS-CoV-2 antigen. The sensor demonstrated excellent performance in assessing and screening COVID-19 and can be used to accurately verify diagnostic data while remaining rapid, simple, and inexpensive.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sabine Szunerits et al.
Summary: Electrochemical biosensors are preferred for biomarker sensing due to their high performance, low cost, miniaturization, and wide applicability. However, electrode fouling severely affects the analytical performance of these sensors, leading to reduced sensitivity, detection limit, reproducibility, and overall reliability. This fouling is caused by nonspecific adsorption of different components in the sensing medium, particularly in complex biofluids like blood. Overcoming fouling is crucial for the future development of electrochemical-based diagnostics for protein biomarkers in a point of care format.
BIOELECTROCHEMISTRY
(2023)
Review
Chemistry, Analytical
Saimon M. M. Silva et al.
Summary: The use of reagentless protein-based biosensors has the potential to revolutionize our understanding of biology and personalized health monitoring by directly monitoring molecular analytes in bodily fluids. Currently available molecular sensors are limited in their applicability to relevant biomarkers due to their reliance on specific enzymatic or chemical reactivity. A new class of reagentless protein-based electrochemical sensors has been introduced to overcome this limitation and enable direct measurements of biomarkers in unprocessed biological fluids. This mini-review discusses recent advances in the development of electroanalytical modular biosensors that integrate all the necessary components for autonomous functionality.
Review
Chemistry, Analytical
Susana Campuzano et al.
Summary: This review explores the extensive research efforts in affinity-based wearable electrochemical biosensors using natural and biomimetic receptors. It critically discusses the rationale and application of selected strategies and provides realistic and futuristic perspectives on the technical barriers, challenges, and prospects of these biodevices in daily routines. The review also analyzes the current trends, opportunities, and challenges in affinity-based wearable electrochemical biosensors using natural and biomimetic receptors.
ANALYSIS & SENSING
(2023)
Review
Chemistry, Analytical
Jie Cheng et al.
Summary: The discovery of electroactive nano-biomaterials and the development of flexible electrodes have spurred interest in integrated electrochemical lateral flow immunoassays (eLFIAs), which offer high sensitivity and selectivity. The future potential of electrochemical sensors lies in their automation and intelligence.
Article
Chemistry, Analytical
Wanwisa Deenin et al.
Summary: In this study, an integrated lateral-flow immunoassay with an electrochemical readout (eLFIA) was developed for early-stage detection of LipL32, a protein present only in pathogenic Leptospira species. The eLFIA showed superior performance in terms of a low detectable limit and a broad linear dynamic range. The test strip provided a straightforward and sensitive approach for the diagnosis of leptospirosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Mayreli Ortiz et al.
Summary: This study developed an electrochemical platform for the detection of SNP in the MYH7 gene associated with hypertrophic cardiomyopathies using fingerprick blood samples. The platform uses solid-phase primer elongation and recombinase polymerase amplification to accurately identify SNPs, and only requires a small amount of fingerprick blood for analysis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Quynh Anh Phan et al.
Summary: CRISPR-Dx offers broad enhancements compared to traditional nanobiosensors by utilizing the trans-cleavage activity of the CRISPR/Cas systems. However, the current singlestranded DNA/RNA reporters of CRISPR-Dx have poor stability and limited sensitivity. Research is shifting towards using the trans-cleavage activity of CRISPR/Cas effectors on metal nanoparticles to develop novel nanobiosensors with high accuracy, sensitivity, selectivity, and versatility.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Biotechnology & Applied Microbiology
Sofia Arshavsky-Graham et al.
Summary: Since the invention of the first biosensors 70 years ago, they have become valuable tools for various applications. Antibodies have traditionally been used as capture probes in biosensors, but suffer from limitations such as size, conjugation chemistry, stability, and cost. Aptamers have emerged as promising alternatives, offering advantages over antibodies and introducing new biosensing concepts.
ENGINEERING IN LIFE SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Gozde Kabay et al.
Summary: This article discusses the challenges and demands in managing viral infectious diseases, and introduces the latest biosensor technologies. It analyzes their advantages and limitations from a material perspective. The development direction of next-generation biosensing technologies is also explored, along with suggestions for further improvement in managing future pandemics.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Analytical
Yao Wu et al.
Summary: This study expands the ability to monitor molecular concentrations by using microneedle-supported electrochemical, aptamer-based (E-AB) sensors in interstitial fluid (ISF). The promising results obtained in vitro and in vivo lay the foundation for the translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Rebeca M. Torrente-Rodriguez et al.
Summary: The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in infected and non-infected individuals is reported. These biotools are built on the surface of magnetic microbeads functionalized with nucleocapsid and recombinant spike proteins. They allow identification of vulnerable populations, monitoring of infection, evaluation of vaccine efficiency, and identification of the variant responsible for the infection.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Aylen Di Tocco et al.
Summary: Research on immunoassays and immunosensors focuses on developing strategies to amplify signals. This study introduces an electrochemical immunosensor that uses viral particles and nanocrystals to conduct more simplified, sensitive, and reliable immunoassays. The developed immunosensor successfully detects molinate in river water samples with high accuracy and excellent recovery rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Materials Science, Multidisciplinary
Susana Campuzano et al.
Summary: Electrochemical biosensors are rapidly advancing in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, thanks to the design and development of nanostructured or multifunctional nucleic acid or peptide biomaterials. This perspective article critically discusses and overviews the recent progress in electrochemical biosensors involving these biomaterials, providing insights into potential and opportunities. The challenges and future directions are also highlighted.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Chemistry, Multidisciplinary
Sasya Madhurantakam et al.
Summary: Rapid diagnosis of COVID-19 is crucial for controlling its spread, and electrochemical sensor platforms show great potential for detecting COVID-19 biomarkers. Point-of-care sensing allows for convenient detection and quantification of health biomarkers, and electrochemical biosensors play a significant role in this field. Integrating electrochemistry with COVID-19 diagnosis and screening using point-of-care technologies enables portable, user-friendly, and rapid analysis.
Article
Materials Science, Multidisciplinary
Midori Johnston et al.
Summary: This study proposes an electrochemical microfluidic multiplexed polymer-based biosensor combined with CRISPR/Cas-powered assays for low-cost and accessible point-of-care nucleic acid testing. The method allows simultaneous screening and identification of SARS-CoV-2 infections while bypassing reverse transcription and target amplification steps. Moreover, the study demonstrates the feasibility of combining different types of synthetic biology-driven assays on the same device, providing a promising on-site tool for rapid diagnosis and disease monitoring.
Article
Biochemical Research Methods
Regina T. Glatz et al.
Summary: Clinical assessment often requires the measurement of multiple analytes or samples to improve the accuracy of diagnosis and therapy. In this study, a microfluidic multiplexed electrochemical biosensor technology (BiosensorX) was developed, allowing simultaneous detection of multiple analytes or samples within a single microfluidic channel. The vertical design of the BiosensorX chip was chosen for its ease of use and superior fluidic behavior. The feasibility of the BiosensorX for on-site testing was demonstrated with the measurement of meropenem antibiotics using an antibody-free beta-lactam assay. This multiplexed biosensor platform can be further extended for the detection of other anti-infective agents and biomarkers, as well as different sample types.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Eva Vargas et al.
Summary: In this study, a theoretical framework for establishing a universal slope for direct calibration-free insulin immunoassays using an electrochemical biosensor was developed. The new method simplifies and speeds up the assay process, reduces costs, and maintains analytical performance without requiring successive standard additions. The results demonstrate the potential for applying this immunoassay protocol to routine decentralized insulin testing.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Alejandro Chamorro-Garcia et al.
Summary: This study develops an electrochemical aptamer-based (EAB) sensor for real-time monitoring of drug levels in the living body. The sensor has wide applicability and high precision with seconds-resolved measurements.
Review
Chemistry, Analytical
Ghasem Ebrahimi et al.
Summary: The integration of microfluidics with electrochemical analysis has led to the development of single miniaturized detection systems with precise control of sample volume and multi-analyte detection capability. Microfluidic electrochemical sensing devices (MESDs) offer several advantages, including automated sample preparation and detection, low sample requirement, and the ability to detect multiple analytes. Label-free MESDs allow for affordable real-time detection and can be used for point-of-care diagnosis and environmental analysis.
Review
Chemistry, Analytical
Qihong Ning et al.
Summary: This article explores the potential of electrochemical biosensors in real-time healthcare monitoring. It discusses the classification and comparison of commonly available electrochemical biosensors, and focuses on recent studies and applications. The article also identifies research gaps and proposes strategies for improvement, providing new guidelines for the advanced research and wider applications of electrochemical biosensors in in vitro diagnosis.
Review
Chemistry, Analytical
Kyeong-Mo Koo et al.
Summary: This article discusses recent advances in the use of electrochemical sensors for determining cell viability and function, emphasizing their importance in pharmaceutical analysis and toxicity testing.
Article
Chemistry, Analytical
Miriam Jauset-Rubio et al.
Summary: Aptamer-based assays and sensors show promise as alternatives to antibodies for their flexibility in assay formats. This study demonstrates the use of a biotin-modified aptamer in enhancing sensitivity and achieving lower detection limits in a colorimetric enzyme-linked oligonucleotide assay and electrochemical detection. The generic nature of this assay format allows for easy and cost-effective application to various aptamers.
Article
Chemistry, Analytical
Sarah Shahub et al.
Summary: This study developed a noninvasive sensor on a flexible, nano-porous substrate to detect cortisol from human sweat passively. A machine learning algorithm was developed to analyze sensor data and detect rising and falling trends of cortisol. The study demonstrated high accuracy and a wide response range for the sensor.
SENSING AND BIO-SENSING RESEARCH
(2022)
Review
Chemistry, Analytical
Gokul Chandra Biswas et al.
Summary: This review examines the development trends of electrochemical POCT tests for the rapid diagnosis of COVID-19 and suggests areas for improvement. It also highlights low-cost and easily affordable setups to aid in future pandemic preparedness.
Article
Chemistry, Multidisciplinary
Eva Vargas et al.
Summary: This study presents a unique design of bioelectronic affinity sensors using natural cell membranes as recognition layers for protein detection and prevention of biofouling. By coating electrochemical transducers with human macrophage membrane as the capture layer and human red blood cell membrane as the nonspecific binding prevention layer, the sensor demonstrates a remarkable limit of detection for tumor necrosis factor alpha (TNF-alpha) cytokine. The integration of natural cell membranes and electronic transduction offers synergistic functionalities for a broad range of biosensing applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Chemistry, Multidisciplinary
Alex M. Downs et al.
Summary: Electrochemical aptamer-based sensors provide a promising approach for real-time molecular monitoring, offering the ability to measure independently of chemical reactivity and supporting in vivo measurements. Despite significant progress, there are still limitations such as short measurement duration and challenges in obtaining high-performance aptamers for new targets.
Review
Biotechnology & Applied Microbiology
Jing Wang et al.
Summary: CRISPR/Cas-based biosensors have been widely used for nucleic acid detection, but their applications for protein detection have been overlooked. Recent studies show that CRISPR/Cas-based biosensors not only analyze proteins, but also enhance the sensitivity and specificity of protein detection. However, there is a lack of comprehensive reviews and elucidation of the mechanisms of CRISPR/Cas-based biosensors for protein detection, which hinders the development of better protein biosensors.
BIOENGINEERING-BASEL
(2022)
Article
Chemistry, Analytical
Dongfei Chen et al.
Summary: An ultrasensitive and highly-selective electrochemical biosensor has been developed for rapid detection of TP53 mutation in blood. Based on a network of gold-coated magnetic nanoparticles modified with probe DNA, the sensor can rapidly and selectively detect TP53 gene with a single-base mutation. The biosensor demonstrates a broad dynamic range and significantly reduced response time.
ANALYSIS & SENSING
(2022)
Article
Chemistry, Multidisciplinary
Fatemeh Keyvani et al.
Summary: This article presents an integrated microfluidic electrochemical assay for cervical cancer detection, which has the potential to identify cervical cancer circulating DNA in whole blood samples. The assay consists of a plasma separator device and a graphene oxide-based electrochemical biosensor, and it has the capability for target DNA detection in whole blood samples, potentially revolutionizing cervical cancer detection and screening in remote areas.
Article
Biotechnology & Applied Microbiology
Lafs C. Lopes et al.
Summary: This article reviews the challenges of designing improved point-of-care (PoC) molecular diagnostic devices and discusses the limitations of standard technologies. It also explores the strategies for developing portable biosensors and reliable commercial PoC devices.
SENSORS AND ACTUATORS REPORTS
(2022)
Review
Chemistry, Analytical
Patrick Severin Sfragano et al.
Summary: Peptides, as a promising class of biorecognition elements, play a significant role in the design and development of electrochemical biosensors. They offer benefits such as stability and selectivity, and can be easily synthesized and modified for use in novel biosensing platforms.
Article
Chemistry, Analytical
Antra Ganguly et al.
Summary: The developed lateral flow electrochemical biosensor uses nanoconfinement technology to rapidly and accurately diagnose urinary tract infections, showing superior specificity and accuracy compared to commercial test strips. This has the potential to improve treatment success rates and reduce costs.
Article
Chemistry, Analytical
Linfei Xiong et al.
Summary: This study developed a DNA dendrimer for loading signal molecules and constructing an amplified electrochemical immunosensing method. The DNA dendrimer was self-assembled by hybridizing complementary oligonucleotides, enabling signal amplification. The method showed excellent performance in analyzing prostate specific antigen as a model analyte.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Stanislav Trashin et al.
Summary: A new method for detecting the antigen marker of human toxocariasis is proposed, utilizing a rapid sandwich immunoassay sensor that achieves high sensitivity to pg/mL levels through a redox cycle involving photocatalytic oxidation and electrochemical reduction steps.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Hatice Ceren Ates et al.
Summary: Integrated diagnostic devices are recognized as the ultimate solution to simplify healthcare testing, accelerating intervention and improving patient outcomes. The combination of non-invasive sampling and integrated devices is of substantial interest for improving patient comfort and safety. Factors affecting the transition of integrated devices from academia to market, such as technology readiness levels and survival rates, are crucial for success.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Analytical
Limin Yang et al.
Summary: This study proposed a highly efficient dual-aptamer recognition system for precise isolation and quantification of tumor exosomes, achieving highly sensitive detection through signal amplification and ratiometric dual-signal strategies. The approach effectively circumvented systematic errors caused by external factors and tedious probe immobilization processes, displaying excellent reliability, improved accuracy, and ease of manipulation.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chawin Srisomwat et al.
Summary: An automated paper-based eLFA device was developed for quantitative detection of hepatitis B virus (HBV) using a time-delayed microfluidic strategy and gold metallization for signal-on electrochemical detection of target DNA. The use of pyrrolidinyl peptide nucleic acid as a probe provided higher specificity and lower background currents, achieving a broad dynamic range and excellent detection limit. The device simplifies the operation for HBV DNA detection without the need for amplification steps, while producing results consistent with standard real-time PCR.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Raquel Sanchez-Salcedo et al.
Summary: A sandwich-type hybridization assay has been developed for detecting prostate cancer by simultaneously measuring prostate cancer antigen 3 and PSA mRNA. This method improves the sensitivity of long noncoding RNA detection and shows good performance in PCa diagnosis without significant cross-talk in the dual configuration.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Derong Kong et al.
Summary: This study presents a new method for COVID-19 nucleic acid detection using Y-shaped DNA dual probes on a graphene field-effect transistor, achieving high recognition ratio and low detection limit. The method enables the fastest nucleic acid testing and the first 5-in-1 pooled testing, with rapid, ultrasensitive, and easily operated features, showing great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Liang Yuan et al.
Summary: Peptides have shown great potential in a variety of applications, particularly in the field of nanotechnology where they are used as biomaterials for constructing new nanostructures. Peptide-based biosensors are being extensively studied and are expected to play a significant role in advanced biomolecular analyses.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Antonia Perju et al.
Summary: Lateral flow assays (LFAs) are considered to be the best point-of-care tests globally, with high performance and recognition. In recent years, there has been increasing interest in researching LFAs, particularly in improving their analytical performance while maintaining their robust detection platform. By integrating electrochemical (EC) methods, significant improvements in LFA performance can be achieved, enabling reliable detection of analytes at lower concentrations than currently possible.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zhen Song et al.
Summary: An antifouling electrochemical biosensor was developed using electropolymerized polyaniline nanowires and newly designed peptides for COVID-19 N-gene detection, demonstrating a low detection limit and wide linear range. The sensor showed high sensitivity and accurate detection capability in complex systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Richard Bruch et al.
Summary: MicroRNAs have become important biomarkers for various diseases in clinical and point-of-care diagnostics, but require consideration of other factors for accurate diagnosis. This study successfully designed and implemented multiplexed versions of an electrochemical microfluidic biosensor for simultaneous quantification of multiple miRNAs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Amanda Clifford et al.
Summary: Portable devices capable of rapid disease detection and health monitoring are crucial to decentralizing diagnostics from clinical laboratories to the patient point-of-need. New work in the field has targeted reagentless approaches to enable breakthroughs that will allow personalized monitoring of a wide range of biomarkers on demand. Reagentless platforms which allow for wash-free, calibration free-detection with increased dynamic range are highlighted as a key technological advance for autonomous sensing applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
David Kodr et al.
Summary: A series of modified nucleoside triphosphates bearing dicarba-nido-undecaborate or metal bis(dicarbollide) groups were prepared and utilized for DNA synthesis and redox labeling. The combination of these redox labels allowed for the first fully orthogonal redox coding of all four canonical nucleobases, enabling determination of nucleobase composition of short DNA stretches with electrochemical readout in one simple primer extension experiment.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Sam A. Spring et al.
Summary: Electrochemical biosensors offer accurate and sensitive analyte detection in opaque samples but are prone to signal drift and error. Inspired by fluorescence methods, scientists have developed ratiometric electrochemical protocols to improve sensor accuracy and reliability in recent years.
Article
Biochemical Research Methods
Julio Aleman et al.
Summary: This article details the integration of sensors on organ-on-chips for continuous monitoring of biomarkers, along with the fabrication and utilization of related technologies.
Article
Chemistry, Analytical
Lin Liu et al.
Summary: This study presents a simple and sensitive electrochemical strategy for detecting telomerase extracted from HeLa cells, utilizing the signal amplification of in-situ formed streptavidin-biotin-DNA-biotin networks. The method effectively amplifies the signal by creating an insulating layer on a gold electrode surface, thus enabling the detection of telomerase.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Materials Science, Multidisciplinary
Heun Park et al.
Summary: The increasing demand for minimally invasive in situ analysis of body fluids has driven rapid development of electrochemically active materials and wearable biosensors. These biosensors can adapt to the body's nonlinearity and integrate with wireless telemetry systems for real-time data display and post analysis. Wearable electronic devices monitoring bodily fluids hold promise for improved management of chronic diseases.
NPG ASIA MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Matthew J. Russo et al.
Summary: Electrochemical biosensors offer a promising approach for rapid on-the-spot sample analysis, but the main issue lies in developing platforms with high sensitivity and selectivity for analyzing low analyte concentrations in complex biological samples, due to nonspecific adsorption at the electrode interface.
Review
Chemistry, Multidisciplinary
Brigitta R. Sun et al.
Summary: Modern smartphone-based sensing devices are standalone detection platforms that can transduce signals, perform analysis through mobile apps, and display results. The development can be divided into three stages, and the mobile app-based detection system is expected to dominate point-of-care diagnosis and on-site molecular sensing in the future.
Review
Chemistry, Multidisciplinary
Shadman Khan et al.
Summary: DNAzymes have been extensively used in biosensors as molecular recognition elements or reporters, with immobilization strategy selection being crucial for sensor accuracy and sensitivity. Various immobilization approaches for DNAzymes within biosensors have been employed, with the selection of an appropriate strategy largely dependent on the desired application.
Review
Chemistry, Analytical
Si-Yuan Wang et al.
Summary: CRISPR/Cas proteins are powerful gene-editing tools and also show great promise in biosensing applications. Various nucleic acid-based signal amplification techniques can greatly improve detection sensitivity and specificity, extend the detectable target range, and enable diverse signal output modes in CRISPR/Cas-based biosensors.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Kenneth R. Wehmeyer et al.
Summary: The development of electrochemical affinity assays and sensors allows for selective and sensitive detection of target analytes, with recent advancements focusing on improving sensitivity and user-friendly features.
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 14, 2021
(2021)
Review
Chemistry, Multidisciplinary
Andreas Frutiger et al.
Summary: The differentiation between specific and nonspecific binding in molecular interactions has been achieved in nature through precise control of biomolecules. However, artificial biosensors have struggled to overcome this issue. Through theoretical models and classification frameworks, it is possible to gain a better understanding and optimize the performance of biosensors, while exploring ways to address the challenge of nonspecific binding.
Article
Chemistry, Multidisciplinary
Richa Pandey et al.
Summary: This study presents an electrical assay that uses electroactive RNA-cleaving DNAzymes to identify specific bacterial targets and release a DNA barcode for rapid clinical analysis with high sensitivity and specificity. The assay demonstrates high selectivity for Escherichia coli and can accurately diagnose bacterial infections in a short amount of time.
Review
Engineering, Biomedical
Kaiyi Zhang et al.
Summary: The emergence and development of noninvasive biosensors have greatly facilitated the collection of physiological signals and health-related data. The utilization of machine learning algorithms enhances the accuracy and efficiency of biosensors, leading to significant advances in healthcare applications.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Materials Science, Biomaterials
Stefanie Fuchs et al.
Summary: Organ-on-chip systems are promising new tools for in vitro research in medical, pharmaceutical, and biological fields, offering improved resemblance to in vivo cell culture environments. Integration of in situ analysis methods on-chip allows for better time resolution and continuous measurements, with sensor technology playing a growing role in enhancing these systems.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Mengdi Bao et al.
Summary: CRISPR, with its unique properties, has emerged as a powerful molecular biosensing tool for nucleic acids and biomarkers. However, several key challenges need to be addressed for it to become a new gold standard. This paper reviews the history of biosensors, discusses current challenges and recent breakthroughs in CRISPR-based nucleic acid detection, and focuses on future advancements needed for CRISPR-based molecular biosensors.
Article
Engineering, Multidisciplinary
Ana Montero-Calle et al.
Summary: This study aimed to identify autoantigens specific to colorectal cancer (CRC) through liquid biopsy for early diagnosis. By analyzing the protein content, a total of 103 potential CRC-specific autoantigens were identified, and further validation showed dysregulation in protein levels of some, along with their association with CRC prognosis. Validation results demonstrated high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals, indicating the potential integration into a point-of-care device for CRC detection.
Review
Engineering, Biomedical
Xun Liu et al.
Summary: Nanomaterial-based electrochemical sensors possess unique properties and compatibility with other techniques, providing opportunities for biomedical applications. Integration with other techniques allows for precise health management and controlled drug release. Future research directions and challenges for commercial implementation of these sensors are presented.
ADVANCED NANOBIOMED RESEARCH
(2021)
Article
Chemistry, Analytical
Alejandro Valverde et al.
Summary: This study developed an electrochemical biosensing platform for detecting serum autoantibodies, utilizing specific peptides as receptors to achieve full diagnostic capability for Alzheimer's disease. The platform was further implemented in a POC multiplexing platform for efficient detection over clinically actionable times.
ANALYSIS & SENSING
(2021)
Article
Chemistry, Multidisciplinary
Ran Liu et al.
Summary: Home testing is an emerging strategy to combat the COVID-19 pandemic and prevent healthcare resource overload. Current diagnostic technologies for SARS-CoV-2 have drawbacks in terms of sensitivity, usability, and costs, making the tests unaffordable for most users at home. To address these limitations, a target-responsive CRISPR-PGM system has been proposed for translating SARS-CoV-2 detection into a glucose test, offering a potential single-device solution for monitoring multiple COVID-19 biomarkers at home.
Article
Chemistry, Multidisciplinary
Dongfei Chen et al.
Summary: An ultrasensitive electrochemical biosensor based on DNA-Au@MNPs has been developed for rapid detection of ctDNA in whole blood, with an ultralow detection limit and broad dynamic range. This sensor provides a promising method for achieving a 20-minute response time and minimally invasive early cancer diagnosis.
Article
Chemistry, Multidisciplinary
Meifang Wang et al.
Summary: The study demonstrates the rapid and sensitive electrochemical detection of doxorubicin using electrochemically pretreated glassy carbon electrodes modified with vertically-ordered mesoporous silica films. The sensor showed ultra-high sensitivity, low limit of detection, wide linear range, and the ability for direct and reliable detection of doxorubicin in human whole blood without complex sample pretreatments, thanks to the excellent anti-fouling and anti-interference properties of the VMSF.
Review
Chemistry, Multidisciplinary
Wei Feng et al.
Summary: The advancement of CRISPR-Cas technology has revolutionized genome editing and transcriptional modulation, as well as molecular detection of diverse targets. Integrating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, improved specificity and sensitivity, and development of point-of-care diagnostic techniques.
Article
Chemistry, Multidisciplinary
Nianzu Liu et al.
Summary: This study developed antifouling electrochemical biosensors based on designed all-in-one branched peptides that combine anchoring, doping, antifouling, and recognizing functions, enabling sensitive and reliable protein quantification in complex serum samples.
CHEMICAL COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Lingyin Meng et al.
BIOTECHNOLOGY ADVANCES
(2020)
Article
Chemistry, Analytical
Alejandro Valverde et al.
Article
Chemistry, Analytical
Lipei Luo et al.
Article
Chemistry, Analytical
Hanmei Deng et al.
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Luke A. Beardslee et al.
Article
Chemistry, Multidisciplinary
Juliane R. Sempionatto et al.
Review
Chemistry, Multidisciplinary
Feiyun Cui et al.
Article
Chemistry, Analytical
Yumeng Liao et al.
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Biophysics
Eva Vargas et al.
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Multidisciplinary
Netzahualcoyotl Arroyo-Curras et al.
Article
Medicine, Research & Experimental
Maria Garranzo-Asensio et al.
Article
Biochemical Research Methods
Kyungyeon Lee et al.
Review
Engineering, Biomedical
Il-Hoon Cho et al.
BIOMATERIALS RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Eva Vargas et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Biophysics
Lin Zhou et al.
BIOSENSORS & BIOELECTRONICS
(2019)
Review
Chemistry, Analytical
Nadeem Baig et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2019)
Review
Chemistry, Multidisciplinary
Can Dincer et al.
ADVANCED MATERIALS
(2019)
Article
Chemistry, Analytical
Jian Lu et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Yingying Tang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xia Li et al.
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Letter
Biotechnology & Applied Microbiology
Richard Bruch et al.
TRENDS IN BIOTECHNOLOGY
(2019)
Article
Chemistry, Analytical
Qian Wang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Biophysics
Magda A. Pereira-Barros et al.
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Multidisciplinary
Shuo Han et al.
Review
Chemistry, Multidisciplinary
Amanda Victorious et al.
FRONTIERS IN CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Beatriz L. Garrote et al.
Article
Chemistry, Analytical
Lele Wang et al.
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Yifan Dai et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Electrochemistry
Raquel Sanchez-Salcedo et al.
Article
Nanoscience & Nanotechnology
Jonathan Sabate del Rio et al.
NATURE NANOTECHNOLOGY
(2019)
Review
Chemistry, Analytical
Alba Iglesias-Mayor et al.
Review
Chemistry, Multidisciplinary
Yong Zhang et al.
Article
Biophysics
Shuopeng Liu et al.
BIOSENSORS & BIOELECTRONICS
(2018)
Article
Electrochemistry
Meijuan Liang et al.
Review
Chemistry, Medicinal
Oleksandra Pashchenko et al.
ACS INFECTIOUS DISEASES
(2018)
Article
Nanoscience & Nanotechnology
Roya Tavallaie et al.
NATURE NANOTECHNOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Victor Ruiz-Valdepenas Montiel et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Engineering, Biomedical
Jayoung Kim et al.
ADVANCED HEALTHCARE MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
Shana O. Kelley
Article
Biophysics
Wei Cai et al.
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Chemistry, Analytical
Victor Ruiz-Valdepenas Montiel et al.
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Prima Dewi Sinawang et al.
BIOSENSORS & BIOELECTRONICS
(2016)
Review
Chemistry, Analytical
Alfredo de la Escosura-Muniz et al.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2016)
Review
Chemistry, Multidisciplinary
Yongxi Zhao et al.
Review
Chemistry, Multidisciplinary
Darius G. Rackus et al.
CHEMICAL SOCIETY REVIEWS
(2015)
Article
Electrochemistry
Maria Bueno Serrano et al.
ELECTROCHEMISTRY COMMUNICATIONS
(2015)
Letter
Biochemical Research Methods
Lloyd M. Smith et al.
Review
Materials Science, Multidisciplinary
Hao Pei et al.
NPG ASIA MATERIALS
(2013)
Review
Chemistry, Multidisciplinary
Alfredo de la Escosura-Muniz et al.
Article
Biophysics
Fernando Javier Arevalo et al.
BIOSENSORS & BIOELECTRONICS
(2012)
Article
Biophysics
Susana Campuzano et al.
BIOSENSORS & BIOELECTRONICS
(2011)
Article
Chemistry, Multidisciplinary
Alfredo de la Escosura-Muniz et al.
Article
Chemistry, Analytical
Jie Wu et al.
ANALYTICAL CHEMISTRY
(2010)
Article
Electrochemistry
Alfredo de la Escosura-Muniz et al.
ELECTROCHEMISTRY COMMUNICATIONS
(2010)