Functional knockout of long non-coding RNAs with genome editing

An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study.

Automated summary

An effective loss-of-function study is necessary to investigate the biological function of lncRNA, and CRISPR-based genome editing is the most widely used method for inactivating lncRNA function at the genomic level. However, the complex genomic interplay between lncRNA and neighbor genes makes it challenging to accurately interpret lncRNA function. This article discusses the advantages and disadvantages of different lncRNA knockout methods and envisions potential future directions for lncRNA functional study.