期刊
FRONTIERS IN IMMUNOLOGY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2023.1286622
关键词
human adenovirus (HAdV); adenoviral vector; adenovirus-based immunization; CD8 T cell response; CD8+T cell response; T cell response; immunogenicity
类别
This study evaluated the effects of 40 different types of adenovirus on activation marker expression, cytokine secretion, and CD8(+) T cell proliferation capacity in dendritic cells. The results showed that different adenovirus types had varying effects on activation marker expression and cytokine secretion, with many types significantly impairing CD8(+) T cell proliferation. Additionally, the study found that type I interferons played an important role in mediating the suppression of CD8(+) T cells.
For the development of new adenovirus (AdV)-based vectors, it is important to understand differences in immunogenicity. In a side-by-side in vitro analysis, we evaluated the effect of 40 AdV types covering human AdV (HAdV) species A through G on the expression of 11 activation markers and the secretion of 12 cytokines by AdV-transduced dendritic cells, and the effect on CD8(+) T cell proliferation capacity. We found that the expression of activation markers and cytokines differed widely between the different HAdV types, and many types were able to significantly impair the proliferation capacity of CD8(+) T cells. Univariate and multivariate regression analyses suggested an important role of type I interferons in mediating this suppression of CD8(+) T cells, which we confirmed experimentally in a proliferation assay using a type I interferon receptor blocking antibody. Using Bayesian statistics, we calculated a prediction model that suggests HAdV types HAdV-C1, -D8, -B7, -F41, -D33, -C2, -A31, -B3 and -D65 as the most favorable candidates for vaccine vector development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据