期刊
FRONTIERS IN IMMUNOLOGY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2023.1148893
关键词
pyoderma gangrenosum (PG); inflammatory bowel disease (IBD); neutrophil extracellular traps (NETs); neutrophilic dermatosis; skin-gut crosstalk; pyrimidine synthesis; skin ulcers
类别
This study describes the development of the first experimental drug-induced mouse model of Pyoderma gangrenosum (PG) with concomitant intestinal inflammation. The mice display skin ulcers enriched in neutrophil extracellular traps (NETs) as well as pro-inflammatory cellular and soluble mediators, mimicking human PG. They also develop spontaneous intestinal inflammation. The novel murine PG model is expected to help researchers probe common disease mechanisms and identify more effective targets for treatment in PG patients with inflammatory bowel disease (IBD).
Pyoderma gangrenosum(PG) is a debilitating skin condition often accompanied by inflammatory bowel disease (IBD). Strikingly, similar to 40% of patients that present with PG have underlying IBD, suggesting shared but unknown mechanisms of pathogenesis. Impeding the development of effective treatments for PG is the absence of an animal model that exhibits features of both skin and gut manifestations. This study describes the development of the first experimental drug-induced mouse model of PG with concomitant intestinal inflammation. Topical application of pyrimidine synthesis inhibitors on wounded mouse skin generates skin ulcers enriched in neutrophil extracellular traps (NETs) as well as pro-inflammatory cellular and soluble mediators mimicking human PG. The mice also develop spontaneous intestinal inflammation demonstrated by histologic damage. Further investigations revealed increased circulating low density IL-1 beta primed neutrophils that undergo enhanced NETosis at inflamed tissue sites supported by an increase in circulatory citrullinated histone 3, a marker of aberrant NET formation. Granulocyte depletion dampens the intestinal inflammation in this model, further supporting the notion that granulocytes contribute to the skin-gut crosstalk in PG mice. We anticipate that this novel murine PG model will enable researchers to probe common disease mechanisms and identify more effective targets for treatment for PG patients with IBD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据