期刊
AMB EXPRESS
卷 13, 期 1, 页码 -出版社
SPRINGER
DOI: 10.1186/s13568-023-01609-9
关键词
Biodegradation; Isoproturon; Catechol 1,2-dioxygenase; qPCR; Pseudomonas putida; catA gene
This study aimed to examine the soil microbial diversity and find bacterial isolates capable of efficiently degrading the persistent phenylurea herbicide isoproturon. The best isolates were identified as Pseudomonas putida and Acinetobacter johnsonii, and their ability to degrade isoproturon was compared. The catA gene responsible for degradation was cloned and expressed in E. coli, which showed higher degradation efficiency than P. putida. The study also identified intermediate metabolites and demonstrated the potential of these bacteria for bioremediation and biodegradation technology development.
The phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from the environment. This study aimed to examine the soil microbial diversity, searching for local bacterial isolates able to efficiently degrade the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). The best isolates able to effectively degrade IPU were selected, characterized, and identified as Pseudomonas putida and Acinetobacter johnsonii. The catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned, and expressed in E. coli M15. The Expressed E. coli showed high degradation efficiency (44.80%) as analyzed by HPLC after 15 days of inoculation in comparison to P. putida (21.60%). The expression of the catA gene in P. putida and expressed E. coli was measured using quantitative polymerase chain reaction (qPCR). The results displayed a significant increase in the mRNA levels of the catA gene by increasing the incubation time with IPU. Hydrophilic interaction chromatography (HILIC) mass spectrometry analysis revealed that three intermediate metabolites, 1-(4-isopropylphenyl)-3-methylurea (MDIPU), 4-Isopropylaniline (4-IA) and 1-(4-isopropylphenyl) urea (DDIPU) were generated by both P. putida and expressed E. coli. In addition, IPU-induced catA activity was detected in both P. putida and expressed E. coli. The supernatant of both P. putida and expressed E. coli had a significant influence on weed growth. The study clearly exhibited that P. putida and expressed E. coli were capable of metabolizing IPU influentially and thus could be utilized for bioremediation and biodegradation technology development.
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