期刊
NANOMATERIALS
卷 13, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/nano13131950
关键词
antibody; diatom; frustule; immunocomplex
In this study, the luminescent properties of antibody-functionalized diatom biosilica frustules were enhanced for label-free photoluminescence (PL) detection of immunocomplex formation. The addition of metabolically doped germanium (Ge) into the frustules increased the PL emission, resulting in a 2.6-fold increase in intensity upon immunocomplex formation.
Diatoms are single-celled algae that biosynthesize cell walls of biogenic silica called frustules that are intricately patterned at the submicron- and nanoscale. In this study, we amplified the intrinsic luminescent properties of antibody-functionalized diatom biosilica frustules for enhanced, label-free, photoluminescence (PL) detection of immunocomplex formation. It was hypothesized that metabolically doped GeO centers in antibody-functionalized diatom biosilica would enhance PL emission associated with nucleophilic immunocomplex formation. Germanium (Ge) was metabolically inserted into the frustule biosilica by two-stage cell cultivation of the centric diatom Cyclotella sp. The biosilica frustules were isolated by hydrogen peroxide treatment and thermally annealed to convert Ge oxides in the biosilica (0.4 wt% Ge) to luminescent GeO centers. The Ge-doped biosilica frustules were then functionalized with Rabbit Immunoglobulin G (IgG). Upon immunocomplex formation with its complimentary antigen goat anti-Rabbit IgG, the Ge-oxide doped, antibody-functionalized frustule biosilica increased the intensity of PL emission by a factor of 2.6 relative to immunocomplex formation by antibody-functionalized frustule biosilica without Ge. It is proposed that the luminescent GeO centers in the Ge-oxide doped frustule biosilica were more sensitive to radiative recombination than luminescent silanol groups in frustule biosilica without Ge, resulting in a higher PL emission upon immunocomplex formation.
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