期刊
NANOTECHNOLOGY
卷 28, 期 4, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/28/4/045704
关键词
magnetoferritin; peroxidase-like activity; kinetic analysis; visualizing tumour tissues; cobalt-doping
资金
- CAS Key Research Program of Frontier Sciences
- National Natural Science Foundation of China [41330104, 41574062, 41621004]
- National R&D Projects for Key Scientific Instruments [ZDYZ2012-1-01-02]
Magnetoferritin (M-HFn) is a biomimetic magnetic nanoparticle with a human heavy-chain ferritin (HFn) shell, trapping a magnetite (Fe3O4) core that has inherited peroxidase-like activity. In this study, cobalt-doped M-HFn nanoparticles (M-HFn-CoxFe3-xO4) with different amounts of cobalt were successfully synthesized. Experimental results indicate that the controlled doping of a certain amount of cobalt into the magnetite cores of M-HFn nanoparticles enhances its peroxidase-like catalytic activity and efficacy for visualizing tumour tissues. For example, compared with sample Co0 (without cobalt doping), the peroxidase-like activity of the cobalt-doped nanoparticle sample Co60 (with a cobalt doping molar percentage of similar to 34.2%) increases 1.7 times, and has the maximal reaction velocity (V-max) values. Moreover, after a one-step incubation with Co60 nanoparticles, and using the peroxidase substrate 3,3'-diaminobenzidine tetrahydrochloride (DAB) for colour development, the tumour tissues of breast, colorectal, stomach and pancreas tumours showed a deeper brown colour with clear boundaries between the healthy and tumourous cells. Therefore, this suggests that the cobalt-doped magnetoferritin nanoparticles enhance peroxidase activity and tumour tissue visualization.
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