Characteristics of microRNAs in Skeletal Muscle of Intrauterine Growth-Restricted Pigs

microRNAs are a class of small RNAs that have been extensively studied, which are involved in many biological processes and disease occurrence. The incidence of intrauterine growth restriction is higher in mammals, especially multiparous mammals. In this study, we found that the weight of the longissimus dorsi of intrauterine growth-restricted pigs was significantly lower than that of normal pigs. Then, intrauterine growth-restricted pig longissimus dorsi were used to characterize miRNA expression profiles by RNA sequencing. A total of 333 miRNAs were identified, of which 26 were differentially expressed. Functional enrichment analysis showed that these differentially expressed miRNAs regulate the expression of their target genes (such as PIK3R1, CCND2, AKT3, and MAP3K7), and these target genes play an important role in the proliferation and differentiation of skeletal muscle through signaling pathways such as the PI3K-Akt, MAPK, and FoxO signaling pathways. Furthermore, miRNA-451 was significantly upregulated in IUGR pig skeletal muscle. Overexpression of miR-451 in C2C12 cells significantly promoted the expression of Mb, Myod, Myog, Myh1, and Myh7, suggesting that miR-451 may be involved in the regulation of the myoblastic differentiation of C2C12 cells. Our results reveal the role of miRNA-451 in regulating myogenic differentiation of skeletal muscle in pigs with intrauterine growth restriction.

Automated summary

MicroRNAs (miRNAs) are small RNAs involved in various biological processes and diseases. In this study, the weight of intrauterine growth-restricted pig longissimus dorsi was found to be significantly lower than that of normal pigs. RNA sequencing identified 333 miRNAs, of which 26 were differentially expressed. Functional analysis revealed that these differentially expressed miRNAs regulate target genes involved in skeletal muscle proliferation and differentiation. Additionally, miRNA-451 was upregulated in IUGR pig skeletal muscle and promoted myoblastic differentiation in C2C12 cells.