期刊
GENES
卷 14, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/genes14081540
关键词
Paulownia fortunei; CaMs/CMLs; gene family; phytoplasma; calcium treatment; Paulownia witches' broom
In this study, 5 PfCaMs and 58 PfCMLs were identified and their conservation and divergence were analyzed through bioinformatics. Some of these genes may be involved in plants' response to phytoplasma infection and exogenous calcium therapy, indicating their role in abiotic and biotic stress responses. Subcellular localization analysis showed that PfCML10 was located in the cell membrane and nucleus. These findings provide a stronger platform for further investigation of the functions of these genes in trees and their roles in Paulownia witches' broom occurrence.
As significant Ca2+ sensors, calmodulin (CaM) and calmodulin-like proteins (CML), have been associated with a variety of environmental conditions in plants. However, whether CaMs/CMLs are related to the stress of phytoplasma infection has not been reported in Paulownia fortunei. In the current study, 5 PfCaMs and 58 PfCMLs were detected through a genome-wide investigation. The number of EF-hand motifs in all PfCaMs/CMLs varied. Bioinformatics analyses, including protein characteristics, conserved domain, gene structure, cis-elements, evolutionary relationship, collinearity, chromosomal location, post-translation modification site, subcellular localization and expression pattern analyses, represented the conservation and divergence of PfCaMs/CMLs. Furthermore, some PfCaMs/CMLs might be involved in plants' reaction to phytoplasma infection and exogenous calcium therapy, indicating these genes may play a role in abiotic as well as biotic stress responses. In addition, subcellular localization analysis showed that PfCML10 was located in the cell membrane and nucleus. In summary, these findings establish a stronger platform for their subsequent functional investigation in trees and further characterize their roles in Paulownia witches' broom (PaWB) occurrence.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据