期刊
CATALYSTS
卷 13, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/catal13091275
关键词
borage oil; gamma-linolenic acid; Thermomyces lanuginosus lipase (TLL); immobilization; ethanolysis; solvent-free system
In this study, borage oil was used as the main source of GLA to obtain ethyl esters through enzymatic ethanolysis using immobilized enzymes. The results showed that the conversion yield of borage oil ethanolysis catalyzed by the immobilized TLL was similar to the chemical pathway. When used in a solvent-free system, the immobilized biocatalyst achieved a high FAEE yield and could be reused multiple times.
In the present work, borage oil (Borago officinalis) was used as the main source of gamma linolenic acid (GLA) to obtain ethyl esters by enzymatic ethanolysis using immobilized enzymes for its application in the food industry. Commercial Thermomyces lanuginosus lipase (TLL) was compared to chemical ethanolysis in alkaline medium. In addition, TLL was immobilized by adsorption on hydrophobic porous support (Octadecyl-Sepabeads (R)) to compare the results. Fatty acid ethyl ester (FAEE) yields of both reactions were compared under the same conditions (25(degrees)C and 200 rpm) and analyzed by GC-MS. Moreover, the conversion yield for borage oil ethanolysis catalyzed by TLL immobilized on C18-Sepabeads (R) supports was similar to the chemical pathway (93.4% and 99.5%, respectively). When this biocatalyst was used in a solvent-free system (at 40(degrees)C and 200 rpm), it was possible to obtain a high FAEE yield of 84.3% in the first 24 h of reaction. Furthermore, it was possible to re-use the immobilized biocatalyst for the performance of five reaction cycles maintaining 68% of its initial activity. Thus, the use of immobilized enzymes in solvent-free systems is an eco-friendly alternative to obtain GLA ethyl esters for its possible application in cosmetics and food.
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