期刊
CATALYSTS
卷 13, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/catal13111399
关键词
oligosaccharides; beta-mannosidase; transglycosylation reaction; enzyme immobilization
The beta-d-mannopyranoside linkage is commonly found in biological structures and is challenging to synthesize chemically. This study successfully immobilized Cf-beta-Man for the first time and used it in the synthesis of beta-mannosides. The immobilized enzyme showed a higher conversion rate in the synthesis of disaccharides under optimal conditions, opening up new possibilities for the design of engineered enzyme mutants and their immobilization.
The beta-d-mannopyranoside linkage is found in a number of biological structures, in particular, in the core trisaccharide of N-linked glycoproteins, as well as within the antigenic polysaccharides of Salmonella, yeasts, and glycolipids. The construction of this glycosydic bond by chemical approach is very challenging and requires cumbersome protection and activation steps prior to glycosylation. In this context, beta-mannosidase from Cellulomonas fimi (Cf-beta-Man) was immobilized for the first time, and it was employed in the synthesis of beta-mannosides. Cf-beta-Man immobilized on IDA-Co2+-agarose allows the synthesis of the disaccharide, cyanomethyl beta-d-mannopyranosyl-(1 -> 6)-2-acetamido-2-deoxy-1-thio-beta-d-glucopyranoside, with a higher conversion compared to the soluble enzyme (20% vs. 5%) after 6 h under best conditions. This explorative work opens new scenarios concerning the design of engineered Cf-beta-Man mutants and their immobilization in order to obtain a robust and recyclable biocatalyst for applications in chemoenzymatic glycan synthesis.
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