期刊
PLANT REPRODUCTION
卷 -, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1007/s00497-023-00481-8
关键词
2n female gamete; SDR; Centromere location; Full-sib population; Hevea brasiliensis
This study evaluates the MHR of 2n female gametes of rubber tree clone GT1, confirming their derivation from SDR and providing insights into the centromere location. These findings are important for understanding the mechanism of 2n gametogenesis and developing related markers.
The evaluation of maternal heterozygosity restitution (MHR) is vital in identifying the mechanism of 2n gametogenesis and assessing the utilization value of 2n gametes. In this study, three full-sib triploid populations were employed to evaluate the MHR of 2n female gametes of rubber tree clone GT1 and to confirm their genetic derivation. The 2n female gametes of GT1 were derived from second-division restitution (SDR) and transmitted more than half of the parental heterozygosity. In addition, low recombination frequency markers were developed, and four candidate physical centromeres of rubber tree were located for the first time. The confirmation that 2n female gametes of rubber tree clone GT1 are derived from SDR provides insights into the molecular mechanisms of 2n gametogenesis. In addition, the identified centromere location will aid in the development of centromeric markers for the rapid identification of the 2n gametogenesis mechanism.
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