期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-023-02038-3
关键词
Mesenchymal stromal cells; Small extracellular vesicles; Acute lung injury; MicroRNA-223-3p; Inflammation
In this study, it was found that small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSC sEVs) could transfer microRNAs (miRNAs) to attenuate inflammation in lung epithelial cells during acute lung injury (ALI). Animal experiments and in vitro cell experiments confirmed this mechanism. MiR-223-3p was identified as a critical mediator in the regulatory effects induced by MSC sEVs. These findings suggest that MSC sEVs may offer a novel promising strategy for ALI.
BackgroundAcute lung injury (ALI), manifested as strong pulmonary inflammation and alveolar epithelial damage, is a life-threatening disease with high morbidity and mortality. Small extracellular vesicles (sEVs), secreted by multiple types of cells, are critical cellular communication mediators and can inhibit inflammation by transferring bioactive molecules, such as microRNAs (miRNAs). Thus, we hypothesized that sEVs derived from mesenchymal stromal cells (MSC sEVs) could transfer miRNAs to attenuate inflammation of lung epithelial cells during ALI.MethodsC57BL/6 male mice were intratracheally administered LPS (10 mg/kg). Six hours later, the mice were randomly administered with MSC sEVs (40 & mu;g per mouse in 150 & mu;l of saline), which were collected by ultracentrifugation. Control group received saline administration. After 48 h, the mice were sacrificed to evaluate pulmonary microvascular permeability and inflammatory responses. In vitro, A549 cells and primary human small airway epithelial cells (SAECs) were stimulated with LPS with or without MSC sEVs treatment.ResultsIn vitro, MSC sEVs could also inhibit the inflammation induced by LPS in A549 cells and SAECs (reducing TNF-& alpha;, IL-1 & beta;, IL-6 and MCP-1). Moreover, MSC sEV treatment improved the survival rate, alleviated pulmonary microvascular permeability, and inhibited proinflammatory responses (reducing TNF-& alpha;, IL-1 & beta;, IL-6 and JE-1) in ALI mice. Notably, miR-223-3p was found to be served as a critical mediator in MSC sEV-induced regulatory effects through inhibition of poly (adenosine diphosphate-ribose) polymerase-1 (PARP-1) in lung epithelial cells.ConclusionsOverall, these findings suggest that MSC sEVs may offer a novel promising strategy for ALI.
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