期刊
NANOSCALE
卷 8, 期 22, 页码 11418-11425出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr07243c
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资金
- National Research Foundation of Korea [2008-0061891, 2009-00426, 2012R1A1A2042550]
- Nano Material Technology Development Program through the National Research Foundation of Korea
- Ministry of Science, ICT & Future Planning [2012M3A7B4035288]
A novel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) biosensor was developed to resolve problems associated with conventional LFA strips (e.g., limits in quantitative analysis and low sensitivity). In our SERS-based biosensor, Raman reporter-labeled hollow gold nanospheres (HGNs) were used as SERS detection probes instead of gold nanoparticles. With the proposed SERS-based LFA strip, the presence of a target antigen can be identified through a colour change in the test zone. Furthermore, highly sensitive quantitative evaluation is possible by measuring SERS signals from the test zone. To verify the feasibility of the SERS-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The limit of detection (LOD) for SEB, as determined with the SERS-based LFA strip, was estimated to be 0.001 ng mL(-1). This value is approximately three orders of magnitude more sensitive than that achieved with the corresponding ELISA-based method. The proposed SERS-based LFA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simplified manner.
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