期刊
NANOSCALE
卷 8, 期 23, 页码 12002-12012出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr00610h
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资金
- Ministry of Health of the Czech Republic [15e33094A]
- ESF European social fund in the Czech Republic - Investments in Education Development [CZ.1.07/2.3.00/20.0306]
- FWO
- MSMT project [LM2011019]
Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor. The proposed device innovates the strategies for NA imaging and delivery, by providing detection of the intracellular release of non-labeled NAs without affecting cellular processing of the NAs. Our system highlights the potential of nanodiamonds to act not merely as labels but also as non-toxic and non-photobleachable fluorescent bio-sensors reporting complex molecular events.
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