期刊
FRONTIERS IN PLANT SCIENCE
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2023.1270150
关键词
Agrobacterium-mediated transformation; buckwheat; callus; CRISPR/Cas9 system; Green Fluorescent Protein (GFP); GreenGate system; phytoene desaturase (PDS); & beta;-glucuronidase (GUS)
Fagopyrum tataricum is an exceptional crop known for its health benefits and gluten-free properties. Through optimized genetic transformation, the study demonstrates the feasibility of trait enhancement in F. tataricum.
Fagopyrum tataricum (L.) Gaertn. is an exceptional crop known for its remarkable health benefits, high levels of beneficial polyphenols and gluten-free properties, making it highly sought-after as a functional food. Its self-fertilisation capability and adaptability to challenging environments further contribute to its potential as a sustainable agricultural option. To harness its unique traits, genetic transformation in F. tataricum is crucial. In this study, we optimised the Agrobacterium-mediated transformation protocol for F. tataricum callus, resulting in a transformation rate of regenerated plants of approximately 20%. The protocol's effectiveness was confirmed through successful GUS staining, GFP expression, and the generation of albino plants via FtPDS gene inactivation. These results validate the feasibility of genetic manipulation and highlight the potential for trait enhancement in F. tataricum.
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