Lost in the bloom: DNA-PKcs in green plants

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a protein encoded by the PRKDC gene in humans and plays a crucial role in repairing DNA double-strand breaks (DSBs). Recent studies have revealed that DNA-PKcs has additional functions in the cell beyond DSB repair, including transcriptional regulation, telomere protection and capping, preserving chromosomal integrity, and regulating senescence, apoptosis, and autophagy. Moreover, DNA-PKcs has also been implicated in regulating the innate immune response, and dysregulation of DNA-PKcs has been commonly observed in various types of cancers. Until recently it was believed that DNA-PKcs is not present in plants in general. However, DNA-PKcs is conserved in green plants ranging from microscopic green algae such as Ostreococcus of the chlorophytes to the tallest living trees on earth, Sequoia of the gymnosperms. Interestingly, DNA-PKcs has not been detected in angiosperms, or in basal angiosperms which are considered sister groups to all other flowering plants. The long polypeptide and gene length of DNA-PKcs coupled with errors in genome assembly, annotation, and gene prediction, have contributed to the challenges in detecting and extracting DNA-PKcs sequences in plant lineages. Sequence alignment showed that several amino acids throughout the length of DNA-PKcs are conserved between plants and human, and all the typical domains identified in human DNA-PKcs are also found in DNA-PKcs from green plants suggesting possible structural and functional conservation. Given the highly conserved nature of DNA repair pathways between mammals and plants further highlights the potential significance of DNA-PKcs in plant biology.

Automated summary

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) encoded by the PRKDC gene is essential for repairing DNA double-strand breaks in humans. It also has additional roles in transcriptional regulation, telomere protection, chromosomal integrity, and cellular processes. Dysregulation of DNA-PKcs is commonly observed in cancers. Recent studies have found that DNA-PKcs is conserved in green plants but not in angiosperms or basal angiosperms. Challenges in detecting DNA-PKcs sequences in plants are attributed to its long polypeptide and gene length. Alignment of sequences between plants and humans suggests possible structural and functional conservation, highlighting the potential significance of DNA-PKcs in plant biology.