4.6 Review

Interplay between chromosomal architecture and termination of DNA replication in bacteria

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Summary: The RecBCD helicase-nuclease complex is crucial for accurately completing DNA replication and promoting resection and joining of excess DNA. Chi sequences alter RecBCD activity and localize with crossover hotspots, but their role in chromosome replication is unknown. This study shows that chi induces replication on substrates with convergent forks, which is processive but uncoupled with respect to leading and lagging strand synthesis, and can be suppressed by ter sites. The findings demonstrate that chi switches RecBCD from a degradative to replicative function and differentiate DNA ends created during completion from those created by double-strand breaks.

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Termination of DNA replication at Tus-ter barriers results in under-replication of template DNA

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Summary: The accurate duplication of genomic information is essential for genome stability. In Escherichia coli, replication termination is confined to the replication fork trap region by Tus protein binding to ter sites. When replication forks fuse at Tus-ter complexes, there is under-replication of DNA template, suggesting the need for further enzymatic processing during fork fusion.

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