期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2023.1245299
关键词
Staphylococcus aureus; antigen-specific activation; cytotoxic CD8(+) T cells; DC2.4; dendritic cells; cytotoxicity; lymphocytes
Staphylococcus aureus is a pathogen associated with various diseases and the emergence of antibiotic-resistant strains has raised concerns. Developing vaccines may help overcome these resistant strains, but the ability of S. aureus to internalize into cells poses a challenge. This study explores the potential of CD8(+) T cells to recognize and kill S. aureus-infected dendritic cells, paving the way for CD8(+) T cell-based therapies against S. aureus.
Staphylococcus aureus (S. aureus) is a pathogen associated with a wide variety of diseases, from minor to life-threatening infections. Antibiotic-resistant strains have emerged, leading to increasing concern about the control of S. aureus infections. The development of vaccines may be one way to overcome these resistant strains. However, S. aureus ability to internalize into cells - and thus to form a reservoir escaping humoral immunity - is a challenge for vaccine development. A role of T cells in the elimination of persistent S. aureus has been established in mice but it remains to be established if CD8(+) T cells could display a cytotoxic activity against S. aureus infected cells. We examined in vitro the ability of CD8(+) T cells to recognize and kill dendritic cells infected with S. aureus. We first evidenced that both primary mouse dendritic cells and DC2.4 cell line can be infected with S. aureus. We then generated a strain of S. aureus expressing a model CD8 epitope and transgenic F5 CD8(+) T cells recognizing this model epitope were used as reporter T cells. In response to S. aureus-infected dendritic cells, F5 CD8(+) T cells produced IFN-gamma in an antigen-specific manner and displayed an increased ability to kill infected cells. Altogether, these results demonstrate that cells infected by S. aureus display bacteria-derived epitopes at their surface that are recognized by CD8(+) T cells. This paves the way for the development of CD8(+) T cell-based therapies against S. aureus.
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