期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2023.1270667
关键词
Pseudomonas aeruginosa; two-component system (TCS); sensor histidine kinase; KdpD; AauS; quorum sensing (QS); virulence
This study evaluated the virulence potential of some uncharacterized sensor HK and RR knockouts in P. aeruginosa and demonstrated that KdpD and AauS HK regulate virulence by affecting biofilm formation and motility. Delta kdpD and Delta aauS mutant strains showed reduced biofilm and motility, and increased survival during in vivo infection. The altered expression of the transcriptional regulators and virulence genes elucidated the mechanism by which Delta kdpD and Delta aauS affect virulence in P. aeruginosa.
Pseudomonas aeruginosa is a multidrug-resistant opportunistic human pathogen that utilizes two-component systems (TCSs) to sense pathophysiological signals and coordinate virulence. P. aeruginosa contains 64 sensor histidine kinases (HKs) and 72 response regulators (RRs) that play important roles in metabolism, bacterial physiology, and virulence. However, the role of some TCSs in virulence remains uncharacterized. In this study, we evaluated the virulence potential of some uncharacterized sensor HK and RR knockouts in P. aeruginosa using a Galleria mellonella infection model. Furthermore, we demonstrated that KdpD and AauS HKs regulate virulence by affecting P. aeruginosa biofilm formation and motility. Both Delta kdpD and Delta aauS showed reduced biofilm and motility which were confirmed by restored phenotypes upon complementation. Moreover, Delta kdpD and Delta aauS exhibited increased survival of HeLa cells and G. mellonella during in vivo infection. Altered expression of the transcriptional regulators anR and lasR, along with the virulence genes lasA, pelA, cupA, pqsA, pqsB, pqsC, and pqsD in the mutant strains elucidated the mechanism by which Delta kdpD and Delta aauS affect virulence. These findings confirm that kdpD and aauS play important roles in P. aeruginosa pathogenesis by regulating biofilm formation and motility.
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