期刊
ELIFE
卷 12, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.86090
关键词
CaMKII; holoenzyme; inter-holoenzyme phosphorylation; IHP; unnatural amino acids; kinase signalling; E. coli; HEK cells
类别
The dodecameric protein kinase CaMKII is expressed throughout the body and plays a crucial role in synaptic plasticity and memory. Contrary to previous expectations, our study reveals that subunit exchange is not the main mechanism for the spread of CaMKII activity. Instead, inter-holoenzyme phosphorylation (IHP) is identified as the mechanism for spreading phosphorylation.
The dodecameric protein kinase CaMKII is expressed throughout the body. The alpha isoform is responsible for synaptic plasticity and participates in memory through its phosphorylation of synaptic proteins. Its elaborate subunit organization and propensity for autophosphorylation allow it to preserve neuronal plasticity across space and time. The prevailing hypothesis for the spread of CaMKII activity, involving shuffling of subunits between activated and naive holoenzymes, is broadly termed subunit exchange. In contrast to the expectations of previous work, we found little evidence for subunit exchange upon activation, and no effect of restraining subunits to their parent holoenzymes. Rather, mass photometry, crosslinking mass spectrometry, single molecule TIRF microscopy and biochemical assays identify inter-holoenzyme phosphorylation (IHP) as the mechanism for spreading phosphorylation. The transient, activity-dependent formation of groups of holoenzymes is well suited to the speed of neuronal activity. Our results place fundamental limits on the activation mechanism of this kinase.
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