期刊
NANOSCALE
卷 8, 期 7, 页码 4203-4208出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr09175f
关键词
-
类别
资金
- National Key Basic Research Program of China [2013CB933704, 2013CB932703]
- National Natural Science Foundation of China [21273240, 11404333, 31571026]
We have designed a novel peptide-Au cluster probe to specifically bind to alpha(IIb)beta(3) integrin. As indicated by molecular dynamics (MD) simulations, the binding mode of the native ligand of alpha(IIb)beta(3) integrin, gamma C peptide, can be realized by the designed probe. More importantly, the peptide-Au probe can provide multiple coating peptides to form additional salt bridges with protein, and the binding stability of the probe is comparable to the native ligand. The designed probe was then successfully synthesized. The specific binding in a cellular environment was validated by colocalization analysis of confocal microscopy. In addition, the binding affinity was confirmed by atomic force microscopy (AFM) based single molecule force spectroscopy. Our results suggest the combination of computational design and experimental verification can be a useful strategy for the development of nanoprobes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据