期刊
NANOSCALE
卷 8, 期 16, 页码 8553-8558出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5nr08232c
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资金
- National Important Science Research Program of China [2011CB933503, 2013CB733800]
- National Natural Science Foundation of China [31170959, 81571806]
- National High Technology Research and Development Program of China [2013AA032205]
- Jiangsu Provincial Special Program of Medical Science [BL2013029]
- Jiangsu Provincial Technical Innovation Fund for Scientific and Technological Enterprises [SBC201310643]
Conventional immunohistochemistry is limited to subjective judgment based on human experience and thus it is clinically required to develop a quantitative immunohistochemical detection. 3,3'-Diaminobenzidin (DAB) aggregates, a type of staining product formed by conventional immunohistochemistry, were found to have a special optical property of dark-field imaging for the first time, and the mechanism was explored. On this basis, a novel immunohistochemical method based on dark-field imaging for detecting HER2 overexpressed in breast cancer was established, and the quantitative analysis standard and relevant software for measuring the scattering intensity was developed. In order to achieve a more sensitive detection, the HRP (horseradish peroxidase)-labeled secondary antibodies conjugated gold nanoparticles were constructed as nanoprobes to load more HRP enzymes, resulting in an enhanced DAB deposition as a dark-field label. Simultaneously, gold nanoparticles also act as a synergistically enhanced agent due to their mimicry of enzyme catalysis and dark-field scattering properties.
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