4.7 Article

Natural and recombinant bioactive compounds from Schizochytrium sp.: Recent advances and future prospects

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biotechnology & Applied Microbiology

Metabolic analysis of Schizochytrium sp. mutants with high EPA content achieved with ARTP mutagenesis screening

Xinyu Wei et al.

Summary: In this study, a mutant strain M12 with improved EPA production efficiency was obtained through ARTP mutagenesis, resulting in a 108% increase in EPA yield reaching 0.48 g/L, and a 13.7% increase in total fatty acid concentration. Transcriptome analysis revealed 2995 differentially expressed genes between M12 and the wild-type strain, with up-regulation of genes involved in carbohydrate, amino acid, energy, and lipid metabolism. Additionally, certain genes related to EPA synthesis were also up-regulated, potentially leading to increased cell growth. These findings provide important insights for further research on fatty acid and EPA accumulation in Schizochytrium sp.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2023)

Article Biochemistry & Molecular Biology

Trypanosoma cruzi Tc24 Antigen Expressed and Orally Delivered by Schizochytrium sp. Microalga is Immunogenic in Mice

Abel Ramos-Vega et al.

MOLECULAR BIOTECHNOLOGY (2023)

Article Biotechnology & Applied Microbiology

Metabolomic analysis reveals the responses of docosahexaenoic-acid-producing Schizochytrium under hyposalinity conditions

Liang Dong et al.

Summary: This study found that under low-salt conditions, the biomass accumulation of Schizochytrium decreased while starch and fatty acid accumulation, especially unsaturated fatty acids, increased. Metabolomic analysis showed that the intracellular pool sizes of NADPH, CoA, and ADP-GCS significantly increased, while those of PEP and AcCoA decreased, consistent with the changes in fatty acid, starch, and biomass content under low-salt stress. The study also discovered that Schizochytrium adapted to low-salt growth conditions by decreasing glycerol content and increasing cell wall polysaccharides. These findings provide important guidelines for low-salt cultivation of Schizochytrium in the future.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2023)

Article Agricultural Engineering

Overexpression of S-R enhances the accumulation of biomass, fatty acids, and 0-carotene in Schizochytrium

Kongyong Lu et al.

Summary: Overexpression of S-R protein in Schizochytrium leads to improved cell growth and significant increase in total fatty acid content, as well as upregulated production of 0-carotene. The overexpression of S-R protein also enhances hypersaline tolerance in Schizochytrium, resulting in increased dry cell weight and production of glycerol and proline. This study provides a feasible strategy for engineering Thraustochytriaceae for efficient biomass and biochemical production.

BIORESOURCE TECHNOLOGY (2023)

Article Biotechnology & Applied Microbiology

Zinc Finger Protein LipR Represses Docosahexaenoic Acid and Lipid Biosynthesis in Schizochytrium sp.

Xiao Han et al.

Summary: In this study, LipR, a C2H2 zinc finger protein, was found to be an essential repressor of DHA and saturated fatty acid biosynthesis in Schizochytrium sp. LipR directly inhibits the transcription of PUFA synthase and fas genes through binding to a specific recognition sequence in the upstream regions of these genes. LipR also represses the expression of genes related to precursor and NADPH levels, as well as the triacylglycerol storage rate. This study provides insights into the regulatory mechanisms of DHA and lipid production in Schizochytrium sp. and suggests that manipulating the lipR gene could enhance the accumulation of polyunsaturated fatty acids and lipids in thraustochytrids.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2022)

Article Agricultural Engineering

A novel two-stage heterotrophic cultivation for starch-to-protein switch to efficiently enhance protein content of Chlorella sp. MBFJNU-17

Xuehua Xiao et al.

Summary: The study established an efficient two-stage cultivation process to produce microalgal biomass rich in protein by optimizing nutrient conditions and gene expression. Chlorella sp. MBFJNU-17 showed promising potential for producing high-value biomass enriched in protein.

BIORESOURCE TECHNOLOGY (2022)

Article Energy & Fuels

Microalgae biomass as a sustainable source for biofuel, biochemical and biobased value-added products: An integrated biorefinery concept

Sk Yasir Arafat Siddiki et al.

Summary: Microalgae biomass has been proven to be a sustainable source for biofuels such as bio-oil, biodiesel, bioethanol, biomethane, etc. The integration of microalgal technologies in industry has the added benefit of capturing carbon dioxide and reducing emissions. While industrial microalgae applications face challenges of energy and cost, utilizing a bio-refinery approach to extract components from microalgae could be a solution. Suitable microalgae species are crucial for high-quality biofuel and value-added products production, with potential applications in various industries such as agriculture, animal husbandry, medicine, culinary, and cosmetics.
Article Biotechnology & Applied Microbiology

Genetic regulation and fermentation strategy for squalene production in Schizochytrium sp.

Qinghua Yang et al.

Summary: This study successfully increased the production of Squalene in Schizochytrium sp. using a combination of metabolic engineering and fermentation strategy. By overexpressing the genes SQS and HMGR, and prolonging the logarithmic growth phase, the content of Squalene was significantly increased.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022)

Article Energy & Fuels

Lipid Productivity and Biosynthesis Gene Response of Indigenous Microalgae Chlorella sp. T4 Strain for Biodiesel Production Under Different Nitrogen and Phosphorus Load

S'fiso T. Gumbi et al.

Summary: This study cultivated Chlorella sp. T4 under different nutrient conditions and found that nitrogen limitation resulted in higher lipid productivity and gene expression, indicating that nutrient limitation can enhance lipid production and biodiesel production.

BIOENERGY RESEARCH (2022)

Review Biotechnology & Applied Microbiology

Lab-scale photobioreactor systems: principles, applications, and scalability

Philipp Benner et al.

Summary: This review discusses different designs of laboratory-scale photobioreactors and provides recommendations for the selection and operation of photobioreactors, as well as scaling up phototrophic bioprocesses with microalgae or cyanobacteria.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Accumulation and conversion of beta-carotene and astaxanthin induced by abiotic stresses in Schizochytrium sp.

Ling Li et al.

Summary: This study explored the effect of different abiotic stresses on carotenoid production and found that low temperature, ferric ion, glucose and monosodium glutamate ratio, and micro-oxygen supply conditions can all regulate the accumulation of carotenoids.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis

Xiuyang Tang et al.

Summary: This study identified the key enzymes for carotenoid biosynthesis in Schizochytrium sp. through bioinformatics analysis. By overexpressing these enzymes, they successfully synthesized 8-carotene, zeaxanthin, and astaxanthin. These findings are significant for understanding the metabolic mechanism in Schizochytrium sp. and for the production of astaxanthin.

ENZYME AND MICROBIAL TECHNOLOGY (2022)

Article Biotechnology & Applied Microbiology

Lipidomic and transcriptomic analysis reveals the self-regulation mechanism of Schizochytrium sp. in response to temperature stresses

Xuechao Hu et al.

Summary: This study investigated the response mechanism of Schizochytrium sp. to temperature stress using transcriptomic and lipidomic approaches. The results showed that high or low temperature inhibited cell growth and affected lipid biosynthesis. Low temperature induced more saturated fatty acids, while high temperature enlarged cell size. Phospholipids were found to be more sensitive to temperature change. The transcriptome analysis revealed the up-regulation of desaturase at low temperature and the up-regulated heat shock proteins at high temperature.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2022)

Article Biotechnology & Applied Microbiology

Optimized production of a bioactive human recombinant protein from the microalgae Chlamydomonas reinhardtii grown at high density in a fed-batch bioreactor

Yasin Torres-Tiji et al.

Summary: Microalgae have been recognized as an alternative platform for producing high-quality biomass and bioproducts. This study examines the potential of the microalga Chlamydomonas reinhardtii to produce a complex human recombinant protein in a high-density heterotrophic culture. The results show that C. reinhardtii is a viable option for producing complex recombinant proteins at high concentrations.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2022)

Article Energy & Fuels

Ultrasound-assisted extraction ameliorates the physicochemical properties of defatted mulberry seed protein to promote lipid production in Schizochytrium sp. SR21

Wen-Jing Li et al.

Summary: Ultrasound-assisted extraction significantly improved the protein extraction rate and modified the properties of mulberry seed protein, enhancing lipid production in Schizochytrium sp. SR21. The treatment also enhanced the antioxidant activity of protein hydrolysate, demonstrating the potential of using mulberry seed protein hydrolysate as a nitrogen source in biorefinery applications.

BIOMASS CONVERSION AND BIOREFINERY (2021)

Article Biotechnology & Applied Microbiology

Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources

Baoyan Gao et al.

Summary: T. bernardii showed rapid growth and high lipid accumulation in heterotrophic culture, making it a potential candidate for biomass and biofuel production. Transcriptome analysis revealed that multilevel regulation ensured the conversion from carbon to the synthesis of carbohydrate and lipid. As nitrogen was consumed, nitrogen metabolism-related genes were up-regulated to speed up the N metabolic cycle, and storage of carbohydrate mainly came from absorption of organic carbon source.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biotechnology & Applied Microbiology

Investigating the effects of eleven key physicochemical factors on growth and lipid accumulation of Chlorella sp. as a feedstock for biodiesel production

Roya Parichehreh et al.

Summary: This study identified Chlorella sp. PG96 as a potential candidate for biodiesel production through a factorial design screening process. The results showed that high light intensity and temperature were more effective factors for algal growth. Additionally, nitrogen and carbon sources played significant roles in biomass and lipid production.

JOURNAL OF BIOTECHNOLOGY (2021)

Review Biotechnology & Applied Microbiology

Advantages of Heterotrophic Microalgae as a Host for Phytochemicals Production

Surumpa Jareonsin et al.

Summary: Currently, most commercial recombinant technologies rely on host systems, each with its own benefits and drawbacks depending on the target products. Prokaryote hosts lack key mechanisms for producing eukaryotic products like phytochemicals, while other eukaryote hosts have limitations such as low yield and high cost. Using heterotrophic microalgae as a host system may offer a solution to these challenges and could be a promising direction for high-value phytochemicals production in the future.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Microalgae-made vaccines against infectious diseases

Abel Ramos-Vega et al.

Summary: Microalgae have come of age as a host for vaccines production and delivery, with several advantages in producing functional antigens for infectious diseases affecting human and animal health. The successful production of various antigens has opened new avenues for research and future perspectives in this field.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2021)

Article Biotechnology & Applied Microbiology

Overproduction of docosahexaenoic acid in Schizochytrium sp. through genetic engineering of oxidative stress defense pathways

Xiao Han et al.

Summary: Enhancing oxidative stress defense pathways through overexpression of TRXR, ALDH, GPO, and ZWF enzymes promotes cell growth, lipid yield, and DHA production in Schizochytrium sp. The engineered strain OaldH-gpo-trxR achieved significantly higher values for dry cell weight, lipid yield, and DHA production compared to the wild type strain. The findings demonstrate the effectiveness of engineering oxidative stress defense pathways in promoting lipid yield and DHA production in Schizochytrium.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biotechnology & Applied Microbiology

Heterotrophically Ultrahigh-Cell-Density Cultivation of a High Protein-Yielding Unicellular Alga Chlorella With a Novel Nitrogen-Supply Strategy

Quan Xu et al.

Summary: In this study, a high-protein Chlorella sorokiniana strain CMBB276 was selected from five Chlorella strains by evaluating their growth rates, protein contents, and yields. Through optimizing the cultural conditions, ultrahigh-cell-density cultivation of C. sorokiniana CMBB276 was achieved, with the highest biomass concentration and protein yield reaching 232 and 86.55 g l(-1) respectively, indicating the great potential for economic and large-scale protein source production.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

Article Nutrition & Dietetics

A Two-Stage Adaptive Laboratory Evolution Strategy to Enhance Docosahexaenoic Acid Synthesis in Oleaginous Thraustochytrid

Sen Wang et al.

Summary: This study employed a two-stage adaptive laboratory evolution (ALE) strategy to enhance the DHA production in Thraustochytrid Aurantiochytrium sp., resulting in the successful obtaining of the end-point strain E-81 with high DHA content. The increased lipid accumulation and DHA content in E-81 strain were attributed to the up-regulated expression of key enzymes involved in lipid accumulation and polyunsaturated fatty acid synthase, respectively.

FRONTIERS IN NUTRITION (2021)

Article Nutrition & Dietetics

Development of an Efficient Gene Editing Tool in Schizochytrium sp. and Improving Its Lipid and Terpenoid Biosynthesis

Peng-Wei Huang et al.

Summary: In this study, an efficient genetic editing tool was developed in Schizochytrium sp. HX-308, leading to an increase in terpenoid production. The results demonstrate the potential of A. tumefaciens-mediated transformation for genetic studies and industrial applications in lipid compound biosynthesis.

FRONTIERS IN NUTRITION (2021)

Article Biotechnology & Applied Microbiology

` Adaptive evolution of microalgae Schizochytrium sp. under high temperature for efficient production of docosahexaeonic acid

Xuechao Hu et al.

Summary: Adaptive laboratory evolution was conducted on Schizochytrium sp. to enhance strain thermotolerance and docosahexaeonic acid (DHA) production, resulting in higher growth rate and lower temperature sensitivity of the adaptive strain. This work successfully increased fermentation temperature by 5 degrees without affecting DHA production, reducing industrial energy consumption cost significantly.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2021)

Article Biotechnology & Applied Microbiology

The chloroplast genetic engineering of a unicellular green alga Chlorella vulgaris with two foreign peptides co-expression

Kang Wang et al.

Summary: This study established a chloroplast transformation system in Chlorella vulgaris for expressing recombinant proteins, providing an efficient genetic tool for further research and application in algae.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2021)

Article Biotechnology & Applied Microbiology

A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography

Daniela Barcenas-Perez et al.

Summary: This study developed a multiple sequential injection HPCCC separation method to isolate DHA ethyl ester and DPA n-6 ethyl ester with high purity, demonstrating potential for coproduction of valuable compounds from microalgae oil to enhance the economics of the approach.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2021)

Article Biotechnology & Applied Microbiology

Function of ORFC of the polyketide synthase gene cluster on fatty acid accumulation in Schizochytrium limacinum SR21

Yanyan Shi et al.

Summary: This study investigated the function of ORFC and its domains in fatty acid accumulation using heterologous expression and overexpression. The results showed that the DH and ER domains played distinct roles in lipid accumulation in Schizochytrium limacinum SR21, with DH domain crucial for PUFA synthesis and ER domain potentially related to SFA synthesis. Metabolic and transcriptomic analysis indicated potential genetic modification strategies for improving lipid production and regulating PUFA and SFA content.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Biotechnology & Applied Microbiology

Microalgal biomass as a biorefinery platform for biobutanol and biodiesel production

Gonzalo M. Figueroa-Torres et al.

BIOCHEMICAL ENGINEERING JOURNAL (2020)

Article Biochemistry & Molecular Biology

Expression in algae of a chimeric protein carrying several epitopes from tumor associated antigens

Jesus Hernandez-Ramirez et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Review Biotechnology & Applied Microbiology

Current advances in the algae-made biopharmaceuticals field

Sergio Rosales-Mendoza et al.

EXPERT OPINION ON BIOLOGICAL THERAPY (2020)

Article Biochemistry & Molecular Biology

High-Density pH-Auxostat Fed-Batch Culture of Schizochytrium limacinum SR21 with Acetic Acid as a Carbon Source

Muhammad Shafiq et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2020)

Article Green & Sustainable Science & Technology

Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101

Jung-Hyun Ju et al.

RENEWABLE ENERGY (2020)

Review Biochemistry & Molecular Biology

The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals

Sergio Rosales-Mendoza et al.

MOLECULES (2020)

Proceedings Paper Engineering, Biomedical

Biogenic Nanoparticle Synthesis Using Marine Alga Schizochytrium sp.

Tugce Mutaf et al.

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON MEDICAL AND BIOLOGICAL ENGINEERING, CMBEBIH 2019 (2020)

Article Food Science & Technology

Antioxidant Assessment of Schizochytrium Meal Protein Enzymatic Hydrolysate and Its Potential Application

Qian Yang et al.

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY (2019)

Article Biotechnology & Applied Microbiology

Microalgae of interest as food source: Biochemical composition and digestibility

Alberto Niccolai et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2019)

Review Biochemistry & Molecular Biology

The lipid metabolism in thraustochytrids

Christian Morabito et al.

PROGRESS IN LIPID RESEARCH (2019)

Review Biotechnology & Applied Microbiology

Nitrogen-dependent coordination of cell cycle, quiescence and TAG accumulation in Chlamydomonas

Tomomi Takeuchi et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Article Chemistry, Physical

Production of H2 by steam reforming in schizochytrium algae oil of cell disruption and extraction via ultrasound method

Xiaohong Hao et al.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY (2019)

Article Biotechnology & Applied Microbiology

Efficient heterotrophic cultivation of Chlamydomonas reinhardtii

Zhen Zhang et al.

JOURNAL OF APPLIED PHYCOLOGY (2019)

Article Biotechnology & Applied Microbiology

Heterotrophic microalgae production on food waste and by-products

Stephan S. W. Ende et al.

JOURNAL OF APPLIED PHYCOLOGY (2019)

Article Biotechnology & Applied Microbiology

Fermentation performance and metabolomic analysis of an engineered high-yield PUFA-producing strain of Schizochytrium sp.

Lingjun Geng et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2019)

Article Engineering, Chemical

Development of a scale-up strategy for fermentative production of docosahexaenoic acid by Schizochytrium sp.

Dong-Sheng Guo et al.

CHEMICAL ENGINEERING SCIENCE (2018)

Article Agriculture, Multidisciplinary

Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp to Improve Polyunsaturated Fatty Acid Production

Zhipeng Li et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2018)

Article Biotechnology & Applied Microbiology

Expression of a Zika virus antigen in microalgae: Towards mucosal vaccine development

Veronica Araceli Marquez-Escobar et al.

JOURNAL OF BIOTECHNOLOGY (2018)

Article Biochemistry & Molecular Biology

Efficient Expression of an Alzheimer's Disease Vaccine Candidate in the Microalga Schizochytrium sp Using the Algevir System

Benita Ortega-Berlanga et al.

MOLECULAR BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Exploring the function of acyltransferase and domain replacement in order to change the polyunsaturated fatty acid profile of Schizochytrium sp.

Lu-jing Ren et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2018)

Article Biotechnology & Applied Microbiology

Functions of PKS Genes in Lipid Synthesis of Schizochytrium sp by Gene Disruption and Metabolomics Analysis

Zhipeng Li et al.

MARINE BIOTECHNOLOGY (2018)

Review Microbiology

Prospects on the Use of Schizochytrium sp to Develop Oral Vaccines

Abel Ramos-Vega et al.

FRONTIERS IN MICROBIOLOGY (2018)

Article Food Science & Technology

Regulation of lipid accumulation in Schizochytrium sp ATCC 20888 in response to different nitrogen sources

Xin Jiang et al.

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2017)

Article Microbiology

Algevir: An Expression System for Microalgae Based on Viral Vectors

Bernardo Banuelos-Hernandez et al.

FRONTIERS IN MICROBIOLOGY (2017)

Article Green & Sustainable Science & Technology

Optimization of biodiesel from dried biomass of Schizochytrium limacinum using methanesulfonic acid-DMC

Balaji M. Panchar et al.

RENEWABLE ENERGY (2016)

Review Biochemistry & Molecular Biology

The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals

Na Yan et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016)

Article Biotechnology & Applied Microbiology

The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.

Xue-Chao Hu et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2015)

Article Agriculture, Multidisciplinary

Introduction of ω-3 Desaturase Obviously Changed the Fatty Acid Profile and Sterol Content of Schizochytrium sp.

Lu-jing Ren et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2015)

Article Agriculture, Multidisciplinary

Preparation and Evaluation of the Chelating Nanocomposite Fabricated with Marine Algae Schizochytrium sp Protein Hydrolysate and Calcium

Jiaping Lin et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2015)

Article Biotechnology & Applied Microbiology

Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition

Lu-Jing Ren et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2014)

Article Agricultural Engineering

Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp.

Lu-Jing Ren et al.

BIORESOURCE TECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Overexpression of acetyl-CoA synthetase increased the biomass and fatty acid proportion in microalga Schizochytrium

Jinfei Yan et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

Impact of phosphate concentration on docosahexaenoic acid production and related enzyme activities in fermentation of Schizochytrium sp.

Lu-Jing Ren et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2013)

Article Engineering, Chemical

Bioprocess Development in Single-Use Systems for Heterotrophic Marine Microalgae

Friederike Hillig et al.

CHEMIE INGENIEUR TECHNIK (2013)

Article Biotechnology & Applied Microbiology

Versatile Transformation System That Is Applicable to both Multiple Transgene Expression and Gene Targeting for Thraustochytrids

Keishi Sakaguchi et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2012)

Article Chemistry, Multidisciplinary

Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion

Jung Kon Kim et al.

KOREAN JOURNAL OF CHEMICAL ENGINEERING (2012)

Article Microbiology

Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium

Rubin Cheng et al.

MICROBIOLOGICAL RESEARCH (2012)

Article Multidisciplinary Sciences

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

James A. Gregory et al.

PLOS ONE (2012)

Review Engineering, Environmental

Heterotrophic cultures of microalgae: Metabolism and potential products

Octavio Perez-Garcia et al.

WATER RESEARCH (2011)

Article Biotechnology & Applied Microbiology

Establishment of a transgene expression system for the marine microalga Schizochytrium by 18S rDNA-targeted homologous recombination

Ru-Bin Cheng et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2011)

Article Biotechnology & Applied Microbiology

Development of a stepwise aeration control strategy for efficient docosahexaenoic acid production by Schizochytrium sp.

Lu-Jing Ren et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2010)

Article Biotechnology & Applied Microbiology

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

Imke A. J. Dreesen et al.

JOURNAL OF BIOTECHNOLOGY (2010)

Review Biotechnology & Applied Microbiology

Inducible gene expression systems and plant biotechnology

Giandomenico Corrado et al.

BIOTECHNOLOGY ADVANCES (2009)

Article Biochemistry & Molecular Biology

Characterization of Both Polyunsaturated Fatty Acid Biosynthetic Pathways in Schizochytrium sp.

J. Casey Lippmeier et al.

LIPIDS (2009)

Review Biochemical Research Methods

Protein production and purification

Susanne Graslund et al.

NATURE METHODS (2008)