期刊
TOXINS
卷 15, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/toxins15080494
关键词
microcystins; biodegradation; linearized microcystinase; purification; activity
The purification and activity of linearized microcystinase (MlrB) were investigated, and the results showed that purified recombinant MlrB could remove the linearized products generated by 12.5 mg/L MC-LR and MC-RR within 30 minutes at a protein concentration of 1 mg/L. This indicates that MlrB has high catalytic activity, laying the foundation for further studies on the properties and mechanism of microcystin biodegradation enzymes.
Hepatotoxic microcystins (MCs) are produced and released by the harmful bloom-forming cyanobacteria, which severely threaten drinking water safety and human health due to their high toxicity, widespread distribution, and structural stability. The linearized microcystinase (MlrB) further hydrolyses the poisonous linearized MCs produced by the microcystinase-catalysed MCs to form tetrapeptides. Here, the purification and activity of MlrB were investigated. The results showed that the linearized products generated by 12.5 mg/L MC-LR and MC-RR were removed by purified recombinant MlrB at a protein concentration of 1 mg/L within 30 min. The high catalytic activity of MlrB can be obtained via heterologous expression and affinity purification, which lays the foundation for further studies on the properties and mechanism of MCs biodegradation enzymes.
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