Effects of Pulsed Electric Field on the Physicochemical and Structural Properties of Micellar Casein

Pulsed electric field (PEF) as a green processing technology is drawing greater attention due to its eco-friendliness and potential to promote sustainable development goals. In this study, the effects of different electric field strengths (EFS, 0-30 kV/cm) on the structure and physicochemical features of casein micelles (CSMs) were investigated. It was found that the particle sizes of CSMs increased at low EFS (10 kV/cm) but decreased at high EFS (30 kV/cm). The absolute & zeta;-potential at 30 kV/cm increased from -26.6 (native CSMs) to -29.5 mV. Moreover, it was noticed that PEF treatment leads to changes in the surface hydrophobicity; it slightly increased at low EFS (10 kV/cm) but decreased at EFS > 10 kV/cm. PEF enhanced the protein solubility from 84.9 (native CSMs) to 87.1% (at 10 kV/cm). PEF at low EFS (10 kV/cm) intensified the emission fluorescence spectrum of CSMs, while higher EFS reduced the fluorescence intensity compared to native CSMs. Moreover, the analysis of the Amide & UIota; region showed that PEF-treated CSMs reduced the & alpha;-helix and increased the & beta;-sheet content. Raman spectra confirmed that PEF treatment > 10 kV/cm buried tyrosine (Tyr) residues in a hydrophobic environment. It was also found that PEF treatment mainly induced changes in the disulfide linkages. In conclusion, PEF technology can be employed as an eco-friendly technology to change the structure and physiochemical properties of CSMs; this could improve their techno-functional properties.

Automated summary

Pulsed electric field (PEF) as a green processing technology can change the structure and physicochemical properties of casein micelles (CSMs), enhancing their techno-functional properties. The effects of different electric field strengths on CSMs were investigated, showing that particle size, surface hydrophobicity, and protein solubility were influenced by PEF treatment. Moreover, PEF treatment affected the fluorescence spectrum and secondary structure of CSMs, as well as disulfide linkages. Overall, PEF technology has the potential to improve CSMs' properties and contribute to sustainable development goals.