Structural and Physical Properties of Alginate Pretreated by High-Pressure Homogenization

To develop a high-efficient extraction method, we investigated the use of high-pressure homogenization (HPH) as a novel pretreatment technology for the extraction of sodium alginate (SA) from Laminaria japonica. After the single-factor experiment, the results demonstrated that under the conditions of 100 MPa HPH pressure, 4 cycles, pH 6.0, and 0.5% EDTA for 3.0 h, the optimized extraction yield of HPH reached 34%. To further clarify the effect on the structural properties of HPH-extracted SA, we conducted comprehensive analysis using SEM, FTIR, MRS, NMR, XRD, TGA, and a T-AOC assay. Our findings revealed that HPH pretreatment significantly disrupted the structure of L. japonica cells and reduced their crystallinity to 76.27%. Furthermore, the antioxidant activity of HPH-extracted SA reached 0.02942 mgVceq & BULL;mg(-1). Therefore, the HPH pretreatment method is a potential strategy for the extraction of alginate.

Automated summary

In order to develop an efficient extraction method, high-pressure homogenization (HPH) was investigated as a novel pretreatment technology for extracting sodium alginate (SA) from Laminaria japonica. The optimized extraction yield of HPH reached 34% under the conditions of 100 MPa HPH pressure, 4 cycles, pH 6.0, and 0.5% EDTA for 3.0 h. HPH pretreatment significantly disrupted the structure of L. japonica cells and reduced their crystallinity to 76.27%. The HPH-extracted SA exhibited an antioxidant activity of 0.02942 mgVceq•mg(-1). Therefore, HPH pretreatment is a potential strategy for alginate extraction.